The present experiment was undertaken to determine the effects of dietary supplements of folic acid and vitamin B12 given from 3 wk before to 8 wk after calving on lactational performance and metabolism of 24 multiparous Holstein cows assigned to 6 blocks of 4 cows each according to their previous milk production. Supplementary folic acid at 0 or 2.6 g/d and vitamin B12 at 0 or 0.5 g/d were used in a 2 x 2 factorial arrangement. Supplementary folic acid increased milk production from 38.0 +/- 0.9 to 41.4 +/- 1.0 kg/d and milk crude protein yield from 1.17 +/- 0.02 to 1.25 +/- 0.03 kg/d. It also increased plasma Gly, Ser, Thr, and total sulfur AA, decreased Asp, and tended to increase plasma Met. Supplementary B12 decreased milk urea N, plasma Ile, and Leu and tended to decrease Val but increased homocysteine, Cys, and total sulfur AA. Liver concentration of phospholipids was higher in cows fed supplementary B12. Plasma and liver concentrations of folates and B12 were increased by their respective supplements, but the increase in plasma folates and plasma and liver B12 was smaller for cows fed the 2 vitamins together. In cows fed folic acid supplements, supplementary B12 increased plasma glucose and alanine, tended to decrease plasma biotin, and decreased Km of the methylmalonyl-coenzyme A mutase in hepatic tissues following addition of deoxyadenosylcobalamin, whereas it had no effect when cows were not fed folic acid supplements. There was no treatment effect on plasma nonesterified fatty acids as well as specific activity and gene expression of Met synthase and methylmalonyl-coenzyme A mutase in the liver. Ingestion of folic acid supplements by cows fed no supplementary B12 increased total lipid and triacylglycerols in liver, whereas these supplements had no effect in cows supplemented with B12. The increases in milk and milk protein yields due to folic acid supplements did not seem to be dependent on the vitamin B12 supply. However, when vitamin B12 was given in combination with folic acid, utilization of the 2 vitamins seems to be increased, probably more so in extrahepatic tissues. Metabolic efficiency seems also to be improved as suggested by similar lactational performance and dry matter intake for cows fed supplementary folic acid but increased plasma glucose and decreased hepatic lipids in cows fed folic acid and vitamin B12 together.

Logging is considered the most important threat to species in boreal forests. In contrast to eastern Canada, where most boreal forests remain largely untouched, in Fennoscandia it is possible to assess the cumulative, long-term effects of intensive forestry on wildlife. But harvesting of stands is rapidly changing Canadian boreal forests, which represent an important proportion of the world's boreal forests. We show that Fennoscandia and eastern Canada present striking similarities in terms of forest-age structure, natural-disturbance regime, and structure of bird assemblages, and we provide an assessment of the long-term effects of forestry on eastern Canadian birds of the boreal forest. We used life-history traits from habitat, nesting site, and geographical range to calculate an index of sensitivity to changes induced by modern forestry for boreal species of each region. Tropical migrants commonly found in eastern Canadian boreal forests have life-history traits that are not threat factors in relation to changes caused by modern forestry. Therefore, the general belief that tropical migrants in North America are more sensitive to landscape changes than those in Europe may not hold for species found in the boreal coniferous forests of eastern Canada. Nine Fennoscandian species present high levels of sensitivity, and at least eight eastern Canadian species are of similar concern. In both regions, most of the sensitive species are resident cavity nesters. Given the important similarities between the two regions, the northern expansion of commercial forestry in eastern Canada is likely to result in the significant decline of several resident species, as has occurred in Fennoscandia. Efectos a LargoPlazo de Actividades Forestales sobre Aves de los Bosques Boreales Canadienses del Este: una Comparación con Fennoscandia Conservation Biology Volume 15, No. 4, August 2001presentan altos niveles de sensibilidad, mientras que por lo menos ochos especies del este de Canadá son de interés similar. En ambas regiones, la mayoría de las especies sensitivas son residentes y anidan en cavidades. Dadas las importantes similitudes entre las dos regiones, es probable que, como en Fennoscandia, la expansión de las actividades forestales comerciales en el este de Canadá resulte en la declinación significativa de varias especies residentes.

Despite intensive recent research on the effects of habitat loss and fragmentation on bird populations, our understanding of underlying demographic causes of population declines is limited. We reviewed avian demography in relation to habitat fragmentation. Then, through a meta-analysis, we compared specific demographic responses by forest birds to habitat fragmentation, providing a general perspective of factors that make some species and populations more vulnerable to fragmentation than others. We obtained data from the scientific literature on dispersal, survival, fecundity, and nesting success of birds. Birds were divided into subgroups on the basis of region, nest site, biogeographical history, and migration strategy. Species most sensitive to fragmentation were ground-or open-nesters nesting in shrubs or trees. Residents were equally sensitive to fragmentation in the Nearctic and Palearctic regions, but Nearctic migrants were more sensitive than Palearctic migrants. Old World species were less sensitive than New World species, which was predicted based on the history of forest fragmentation on these two continents. Pairing success was the variable most associated with fragmentation, suggesting an important role of dispersal. Fledgling number or condition, timing of nesting, and clutch size were not associated with sensitivity to fragmentation, suggesting that negative fragmentation effects on birds do not generally result from diminished food resources with increasing level of fragmentation. Future studies on demographic responses of birds to habitat fragmentation would be more effective if based on a combination of measures that can distinguish among the demographic mechanisms underlying population changes related to habitat fragmentation.Key Words: bird demography, edge effects patch size, habitat loss, meta-analysis, patch isolation Respuestas Demográficas de Aves a la Fragmentación de BosquesResumen: A pesar de la reciente investigación intensiva sobre los efectos de la pérdida y fragmentación del hábitat sobre poblaciones de aves, nuestro entendimiento de las causas demográficas subyacentes en laa declinaciones poblacionales es limitado. Revisamos la demografía aviar en relación con la fragmentación del hábitat. Posteriormente, mediante un meta análisis comparamos las respuestas demográficas específicas de aves de bosque a la fragmentación del hábitat, lo que proporcionó una perspectiva general de los factores que hacen que algunas especies y poblaciones sean más vulnerables a la fragmentación que otras. Obtuvimos datos de la literatura científica sobre dispersión, fecundidad yéxito de anidación de aves. Las aves fueron divididas en subgrupos con base en la región, sitio de anidación, historia biogeográfica y estrategia de migración. Las especies más sensibles a la fragmentación anidaban sobre el suelo oáreas abiertas en matorrales oárboles. Las especies residentes fueron iguamente sensibles a la fragmentacíón en las regiones Neártica y Paleártica, pero los migrantes Neárticos fueron más sensibles que l...

The end-Ordovician was an enigmatic interval in the Phanerozoic, known for massive glaciation potentially at elevated CO 2 levels, biogeochemical cycle disruptions recorded as large isotope anomalies and a devastating extinction event. Ice-sheet volumes claimed to be twice those of the Last Glacial Maximum paradoxically coincided with oceans as warm as today. Here we argue that some of these remarkable claims arise from undersampling of incomplete geological sections that led to apparent temporal correlations within the relatively coarse resolution capability of Palaeozoic biochronostratigraphy. We examine exceptionally complete sedimentary records from two, low and high, palaeolatitude settings. Their correlation framework reveals a Cenozoic-style scenario including three main glacial cycles and higher-order phenomena. This necessitates revision of mechanisms for the endOrdovician events, as the first extinction is tied to an early phase of melting, not to initial cooling, and the largest d 13 C excursion occurs during final deglaciation, not at the glacial apex.

The present experiment was undertaken to determine the effects of dietary supplements of rumen-protected methionine and intramuscular injections of folic acid and vitamin B 12 , given 3 wk before to 16 wk after calving, on glucose and methionine metabolism of lactating dairy cows. Twenty-four multiparous Holstein cows were assigned to 6 blocks of 4 cows each according to their previous milk production. Within each block, 2 cows were fed a diet estimated to supply methionine as 1.83% metabolizable protein, equivalent to 76% of methionine requirement, whereas the 2 other cows were fed the same diet supplemented daily with 18 g of rumen-protected methionine. Within each diet, the cows were administrated either no vitamin supplement or weekly intramuscular injections of 160 mg of folic acid plus 10 mg of vitamin B 12. To investigate metabolic changes at 12 wk of lactation, glucose and methionine kinetics were measured by isotope dilution using infusions of d[U-13 C]glucose, [ 13 C]NaHCO 3 and l[1-13 C, 2 H 3 ] methionine. Milk and plasma concentrations of folic acid and vitamin B 12 increased with vitamin injections. Supplementary B-vitamins increased milk production from 34.7 to 38.9 ± 1.0 kg/d and increased milk lactose, protein, and total solids yields. Whole-body glucose flux tended to increase with vitamin supplementation with a similar quantitative magnitude as the milk lactose yield increase. Vitamin supplementation increased methionine utilization for protein synthesis through increased protein turnover when methionine was deficient and through decreased methionine oxidation when rumen-protected methionine was fed. Vitamin supplementation decreased plasma concentrations of homocysteine independently of rumen-protected methionine feeding, although no effect of vitamin supplementation was measured on methionine remethylation, but this could be due to the limitation of the technique used. Therefore, the effects of these B-vitamins on lactation performance were not mainly explained by methionine economy because of a more efficient methylneogenesis but were rather related to increased glucose availability and changes in methionine metabolism.