Water deficit affects tree growth and limits wood production. In an attempt to identify the molecular triggers of adaptation mechanisms to water deficit in Eucalyptus, we investigated protein expression patterns of two ecophysiologically contrasted Eucalyptus genotypes. They were grown in the field in either natural conditions or irrigated for 7 weeks during the dry season in the Republic of Congo. At the phenotypic level, genotype (G), treatment (T) and/or G ¥ T interaction effects were observed for aboveand below-ground biomass-related traits. At the molecular level, changes in protein abundance were recorded in leaves (acidic pH 4-7, and basic pH 7-11, proteomes) and stems (acidic proteome) using two-dimensional gel electrophoresis (2-DE). One third of the detected protein spots displayed significant G, T and/or G ¥ T effects, and 158 of them were identified by tandem mass spectrometry (LC-MS/MS) analysis. Thus, several proteins whose molecular plasticity was genetically controlled (i.e. G ¥ T effect) were revealed, highlighting adaptive mechanisms to water deficit specific to each genotype, namely cell wall modification, cell detoxification and osmoregulation. Transcript abundances corresponding to G ¥ T proteins were also investigated by quantitative RT-PCR. These proteins represent relevant targets to improve drought resistance in this ecologically and economically important forest tree genus.

Located in the hearth of Africa, the Congo basin is the world’s second largest rainforest ecosystem, spanning over nine countries including the Republic of the Congo. Nature-based solutions, i.e., afforestation, reforestation or agroforestry supplying wood energy, halting food insecurity, restoring land desertification and fostering mitigation and adaptation to climate warming, have been increasingly used in the past decades. Within this framework, Congolese coastal plains have been afforested using fast growing trees since the early 1950s. Due to the low forest productivity and soil fertility, sustainable management of these forest ecosystems (trees, soils and environment) have been performed. Improved germplasms, increased stand wood biomass and healthier soils have the potential to enhance wood and fuel wood energy supply, mitigation and adaptation to climate change, food security, restoration of land and ecosystem biodiversity. This meets ten out of the seventeen sustainable development goals (SDG #), specifically goals related to alleviating poverty (1) and hunger (2), improving health (3), education (4), sanitation and access to clean water (6). Other goals include providing affordable clean energy (7), sustainable production and consumption (12), action on climate change (13), life on land (15), and partnerships for goals (17). Nature-based solutions help to face important societal challenges meeting more than half of SDGs of the United Nations.