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Objective: To evaluate the use of mesenchymal stem cells (MSCs) in the attenuation of canine atopic dermatitis (AD). Materials and methods: Sixteen dogs were selected and divided into three groups, mild, moderate, and severe, according to the Canine Atopic Dermatitis Extent and Severity Index (CADESI-4). They were evaluated for 82 days. The protocol recommended in this experiment was to inject 2 × 10 6 /kg bodyweight of MSC’s in all groups by the intravenous route with intervals of applications of 21 days. The degree of pruritus was evaluated by examining the visual analog scale, the CADESI-4, the histopathology of the skin, hematological and biochemical parameters, the pyogenic effect of MSCs, and the thickness of the epidermis. Results: There was a significant difference in the reduction of epidermal thickness in the moderate and severe groups. Hematological, biochemical, and body temperature parameters remained within normal limits for the species with no side effects Conclusion: MSCs attenuated the clinical signs of AD.

Obesity is transgenerational epigenetic metabolic disturbance. Although the diet-induced obese (DIO) zebrafish model is well established, reproductive parameters and changes in offspring have not yet been evaluated. Thus, the aim of this study was to evaluate possible changes in reproductive parameters, embryos and offspring (F1) generated by the reproduction of diet-induced obese males and females. The adult zebrafish were divided into two groups receiving a balanced diet (Control group) or overfed (DIO group) were investigated. The dietary protocol was maintained for eight weeks. During this period, males and females in the same group were stimulated through a weekly reproduction protocol. To verify parental obesity, body weight, blood glucose, triglyceride, the hepatosomatic and gonadosomatic index and adipose tissue morphometry evaluations were carried out. Reproductive parameters were evaluated through ovarian and oocyte maturation stage, total spawning, fertility and fertilization index. To verify possible changes caused by parenteral obesity, all offspring were kept in separate groups in correspondence with their parents and were fed a control diet. Plasma glucose, triglycerides, mortality rate, hatching, and deformities were determined. After eight weeks under the diet protocol, the DIO group exhibited characteristic obesity alterations, displaying significant increases in body mass and hepatosomatic and gonadosomatic indices, hyperglycemia and visceral and subcutaneous adipocyte hypertrophy. In addition, high mortality rates, morphologic deformities and high plasmatic glucose and triglyceride levels, with 100% mortality at 60 dpf, were observed for the offspring. Therefore, obesity induction in adults led to negative effects on their offspring, with high occurrence of deformities and mortality.

ABSTRACT. Twenty-eight fowls (Gallus gallus domesticus) of the Red Bro Cou Nu lineage were studied. They were about 35 days old and had their arteries filled with Neoprene Latex 450", aqueous solution at 50%, stained with specific pigment (Globo S/A, Tintas e Pigmentos), and then fixed in aqueous solution of formaldehyde at 10%. The present study aimed to quantify the thymic lobes and identify the main arteries responsible for the blood supply of these lobes and their frequencies. The number of thymic lobes on the right antimere varied from two to 11, with a greater frequency of 8 lobes; and the left antimere showed a variation from one to eight, with a greater frequency of five lobes. The arteries destined to the thymus were composed by direct and indirect branches of the following arteries: left and right common vagus nerve, left and right cranial thyroid, left and right caudal thyroid, left and right ascending esophageal, right ingluvial, left and right cutaneous, and right common carotid. The right thymic lobes, regardless their origin, received from eight to 27 vascular branches, and the left thymic lobes, from eight to 24 branches. Keywords: anatomy, irrigation, Gallus gallus.Morfologia macroscópica e suprimento vascular arterial do timo em aves (Gallus gallus domesticus) da linhagem Red Bro Cou Nu RESUMO. Foram estudadas 28 aves (Gallus gallus domesticus) da linhagem Red Bro Cou Nu, com aproximadamente 35 dias de idade, que tiveram suas artérias preenchidas com Látex Neoprene 450", solução aquosa a 50%, corado com pigmento específico (Globo S/A, Tintas e Pigmentos) e em seguida foram fixadas em solução aquosa a 10% de formol. O objetivo do presente estudo foi quantificar os lobos tímicos e identificar as principais artérias responsáveis pelo suprimento sanguíneo arterial e suas frequências. No antímero direito cada cadeia tímica apresentou de dois a 11 lobos tímicos, com maior frequência de oito lobos. O antímero esquerdo apresentou de um a oito lobos tímicos, sendo que a maior frequência foi de cinco lobos. O suprimento arterial do timo das aves da linhagem Red Bro Cou Nu do presente estudo era composto por ramos diretos e indiretos oriundos das artérias: comum do nervo vago direita e esquerda, tireóidea cranial direita e esquerda, tireóidea caudal direita e esquerda, esofágica ascendente direita e esquerda, ingluvial direita, cutânea direita e esquerda e carótida comum direita. Os lobos tímicos direitos receberam independentemente de suas origens, de oito a 27 ramos vasculares e os lobos tímicos esquerdos receberam de oito a 24 ramos.Palavras-chave: anatomia, irrigação, Gallus gallus.