Adenocarcinomas are malignant epithelial neoplasms of glandular nature and have been reported in many organs of companion animals including the lungs, thyroid, prostate, mammary glands, gallbladder, pancreas, esophagus, stomach, and intestine. In felines, reports of such neoplasms in the genitalia and associated glands are very rare and have a reserved prognosis. There are no reports in the veterinary literature describing this type of neoplasia affecting the foreskin of cats. Cytological analysis and histopathological evaluation of incisional or excisional biopsy samples can confirm the diagnosis of preputial neoplasms. The gold standard treatment consists of surgical excision of the neoplasm. In the present report, a 16-year-old male Brazilian shorthair feline was referred to the Surgical Clinic service of the Companion Animal Veterinary Hospital of UFRRJ, with a major complaint of increased volume and ulceration in the preputial and penile region with slow growth. The animal experienced dysuria, urinary retention, and pollakiuria. It had a history of recurrent urinary obstructions and urolithiasis. A penile urethrostomy was performed, and the mass was histopathologically and microbiologically analyzed due to a suspicion of fungal or neoplastic involvement. Histopathological examination showed the presence of tubular adenocarcinomas. Chemotherapy treatment was suggested in the immediate postoperative period, but it was not performed per the owner's request. One year after the foreskin resection and creation of a new urethral stoma, a new mass was found in the perineal region, and the animal died one week later.
This study aimed to evaluate two protocols (PA and PB) that are used to obtain canine platelet-rich plasma (PRP) for cellularity. Twenty healthy dogs were used. Blood samples were collected and placed in two tubes of 3.2% sodium citrate. PA used double centrifugation at 210 x g, and 370 x g and PB used double centrifugation with 140 x g and 330 x g. The PRP samples from the protocols were examined in terms of their platelet, erythrocyte, and leukocyte count in the Neubauer chamber, differential leukocyte count and platelet morphological observation in blood smears. Data (mean and standard deviation) were analyzed with the 95% probability t-test (P <0.05) using Pearson’s correlation to test the relationship between platelets and erythrocytes, platelets, and leukocytes, and the leukocyte count versus the erythrocytes. Very weak negative correlation between platelets and leukocytes (p= -0.03), weak negative correlation between platelets and erythrocytes (p= -0.3) and a strong positive correlation between leukocytes and erythrocytes (ρ = 0.75) were noted. Although, BP did not reach the desired mean of one million platelets (979300 ± 79631 cells / μL), both protocols, A and B (4.42 ± 1.61 and 3.85 ± 1.55 times more platelets than total blood, respectively) (p <0.05) were efficient in concentrating platelets. Platelet activation was present in 26.55 ± 6.72% of the PA platelets and 26.25 ± 7.03% in PB (p> 0.05). PA and PB presented low erythrocyte concentration (p> 0.05), and PA had more leukocytes (p <0.05) than PB, with higher concentrations of basophils that were segmented, and lymphocytes.
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