These authors contributed equally to this work. Humans settled the Caribbean ~6,000 years ago, with intensified agriculture and ceramic use marking a shift from the Archaic Age to the Ceramic Age ~2,500 years ago. To shed new light on the history of Caribbean people, we report genome-wide data from 184 individuals predating European contact from The Bahamas, Cuba, Hispaniola, Puerto Rico, Curaçao, and northwestern Venezuela. A largely homogeneous ceramic-using population most likely originating in northeastern South America and related to present-day Arawak-speaking groups moved throughout the Caribbean at least 1,800 years ago, spreading ancestry that is still detected in parts of the region today. These people eventually almost entirely replaced Archaic-related lineages in Hispaniola but not in northwestern Cuba, where unadmixed Archaic-related ancestry persisted into the last millennium. We document high mobility and inter-island connectivity throughout the Ceramic Age as reflected in relatives buried ~75 kilometers apart in Hispaniola and low genetic differentiation across many Caribbean islands, albeit with subtle population structure distinguishing the Bahamian islands we studied from the rest of the Caribbean and from each other, and long-term population continuity in southeastern coastal Hispaniola differentiating this region from the rest of the island. Ceramic-associated people avoided close kin unions despite limited mate pools reflecting low effective population sizes (2Ne=1000-2000) even at sites on the large Caribbean islands. While census population sizes can be an order of magnitude larger than effective population sizes, pan-Caribbean population size estimates of hundreds of thousands are likely too large. Transitions in pottery styles show no evidence of being driven by waves of migration of new people from mainland South America; instead, they more likely reflect the spread of ideas and people within an interconnected Caribbean world.

Humans settled the Caribbean ~6,000 years ago, with ceramic use and intensified agriculture marking a shift from the Archaic to the Ceramic Age ~2,500 years ago 1 – 3 . We report genome-wide data from 174 individuals from The Bahamas, Hispaniola, Puerto Rico, Curaçao, and Venezuela co-analyzed with published data. Archaic Age Caribbean people derive from a deeply divergent population closest to Central and northern South Americans; contrary to previous work 4 , we find no support for ancestry contributed by a population related to North Americans. Archaic lineages were >98% replaced by a genetically homogeneous ceramic-using population related to Arawak-speakers from northeast South America who moved through the Lesser Antilles and into the Greater Antilles at least 1,700 years ago, introducing ancestry that is still present. Ancient Caribbean people avoided close kin unions despite limited mate pools reflecting small effective population sizes which we estimate to be a minimum of Ne=500–1500 and a maximum of Ne=1530–8150 on the combined islands of Puerto Rico and Hispaniola in the dozens of generations before the analyzed individuals lived. Census sizes are unlikely to be more than ten-fold larger than effective population sizes, so previous estimates of hundreds of thousands of people are too large 5 – 6 . Confirming a small, interconnected Ceramic Age population 7 , we detect 19 pairs of cross-island cousins, close relatives ~75 kilometers apart in Hispaniola, and low genetic differentiation across islands. Genetic continuity across transitions in pottery styles reveals that cultural changes during the Ceramic Age were not driven by migration of genetically-differentiated groups from the mainland but instead reflected interactions within an interconnected Caribbean world 1 , 8 .

Dental caries and antemortem tooth loss (AMTL) are investigated in a Classic Maya sample obtained from the sites of Calakmul, Dzibanché, and Kohunlich (Mexico). This study aims at assessing the effect that sex and social status had on the prevalence of oral pathologies. The lack of a direct relationship between caries, AMTL, and age-at-death led us to interpret the results in terms of the biological, socioeconomic, and behavioral conditions prevailing in these ancient Maya settlements. Benefits related to sex and social status are evident in the frequency of carious lesions, which appear less frequently in elite males than in low-status individuals of both sexes and in elite females. Individuals from problematic mortuary contexts and isolated bone assemblages, who could not be ascribed to any status group, showed the highest rates of caries. Sex discrimination in dietary preferences appears in the elite sample, while the homogeneity encountered between sexes in the low-status segment suggests a more uniform access to resources. Tooth loss clearly distinguishes elite individuals from commoners, regardless of sex, with the former bearing a much higher rate of loss. In individuals from the undefined mortuary assemblages and sacrificial contexts, it was even more pronounced than in the other groups, although its interpretation is problematic due to a lack of associated funerary data. The overall evidence from oral pathologies is interpreted to be the result of deficient oral hygiene coupled with a softer and more refined diet in the high-status population, particularly males. Whereas elite males' subsistence was apparently based more on animal proteins and relatively soft and refined foods, a diet relying on carbohydrates may account for the observed rate of oral pathologies in elite females and commoners.

In AD 2000, construction activities in the central plaza of the city of Campeche, Mexico, led to the discovery of an early colonial church and an associated burial ground dating from the sixteenth and seventeenth centuries AD. During the subsequent rescue excavations, the remains of at least 180 individuals were unearthed from the churchyard. We have concluded a series of isotopic studies of these remains to obtain information on diet, status, place of origin, and date of burial. This work involves the application of both light and heavy isotope analyses to both tooth enamel and human bone. Carbon and oxygen isotope ratios were measured in tooth enamel and bone. Carbon and nitrogen isotope ratios were measured on bone collagen. Strontium and lead isotopes were measured in tooth enamel, and the ratios were compared to a large database for the Maya region. Radiocarbon dates were obtained for 10 of the skeletons to evaluate the date of burial and the period of use of the cemetery. The results of our study, interpreted jointly with mortuary information and conventional skeletal examination, provide detailed information on the overall burial population, a sort of collective life history of the deceased individuals. In the context of the historical background, new insights on living conditions, mobility, and diet of the founding generations in the colonial New World are obtained. A new and direct appreciation on life and death in an early multiethnic colonial Spanish town, including its historically invisible sectors-children, women, servants, and slaves-becomes possible.In the study described here, we examine the isotopic composition of bone and tooth enamel from a number of the individuals buried in an early colonial cemetery in the modern city of Campeche, on the west coast of the Yucatán Peninsula in Mexico. We are interested in questions about diet, status, place of birth, and date of burial. We employ isotopes of carbon, oxygen, nitrogen, lead, and strontium in this study. Carbon isotopes in bone collagen, bone mineral, and tooth mineral have been measured in some of the skeletal remains. We have also measured radiocarbon isotopes in bone collagen for age determinations. Oxygen isotopes have been measured in bone mineral and tooth enamel. Nitrogen isotopes have been measured in bone collagen. Strontium and lead isotope ratios have been assayed in tooth enamel and strontium isotopes in bone mineral. Lead isotope ratios have been measured in a few enamel samples. The isotopic results are examined in light of the contextual evidence of discovery and the biological and biocultural information provided by the analyses of the human remains.The first sections of this article provide some historical background on the early town of Campeche and the early church that was the focus of religious ceremony and sacred burial. A description of the excavations provides the archaeological and taphonomic context of the human burials in the Campeche plaza cemetery. These and conventional bioarchaeological studies provide the basic a...

Prehistoric evidence for the drilling of human teeth in vivo has so far been limited to isolated cases from less than six millennia ago. Here we describe eleven drilled molar crowns from nine adults discovered in a Neolithic graveyard in Pakistan that dates from 7,500-9,000 years ago. These findings provide evidence for a long tradition of a type of proto-dentistry in an early farming culture.