The obtained results demonstrate that hybrid IgE repertoires represent a source for human antibodies with genuine paratopes. The IgE-derived information about the IgE epitope nature of Bet v 1 and homologues allows for detailed insights into molecular aspects of allergenicity and cross-reactivity within the PR10 protein family.

Background: Antibody binding to xenobiotic ␣-Gal structures mediates anaphylaxis. Results: Humanized IgE antibodies exhibit recognition footprints similar to serum immunoglobulins but have no capability of effector cell activation. Conclusion: Recognition of the ␣-Gal epitope is based on the terminal disaccharide, but interaction does not imply cellular activation. Significance: These data provide the first insights into the recognition of carbohydrate IgE epitopes.

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