Aim: Verdicchio is a white wine grape variety that has been cultivated for hundreds of years in the Marche region of central Italy. Verdicchio is used to produce all kinds of dry, sweet and sparkling wines, some of which can be aged for ten or more years. This study aimed to extend knowledge of the volatile profile of Verdicchio wines and the recognition and detection of odorous molecules. We considered wines produced in multiple vintages from some of the best Cru from the Marche region, in the Castelli di Jesi Classico area.Methods and results: Two data sets were considered: a vertical collection that included wines from different vintages, same variety, different production areas vinified by the same winery and a horizontal collection of wines from the 2016 harvest, considering different production areas, harvest times and clones produced by the same winery. Samples were analysed with GC×GC-ToF-MS, GC-MS-MS and GC-O. Comprehensive profiling with more than 1000 compounds allowed the wines produced in different areas to be separated. By GC-O analysis 48 main odorants were found. This survey led to the identification of 3-methyl-2,4-nonanedione (3-MND) that impart an anise note and an interesting content in methyl salicylate as a possible key odorant characteristic in Verdicchio.Conclusion: The volatile profile of different Verdicchio wines from the best production areas was investigated in detail. This work confirms that it is possible to obtain wines with very different characteristics from this variety of grapes, producing premium wines with a distinctive pattern of volatiles, reproducible across several vintages and variable depending on the different location of the vineyard and winemaking techniques. Young wines are characterised by fruity, thiolic notes, while wines aged for longer are distinguished by their norisoprenoids content and by anise and balsamic notes, which can be attributed to the presence of 3-MND and to methyl salicylate released by precursors. With a derivatisation GC-MS-MS method it was possible to quantify 3-MND in the range of 10–50 ng L−1. With the use of GC-O, 48 potentially odour-active compounds were found for this wine. Analysis of the compounds after hydrolysis confirmed that a high amount of methyl salicylate characterised this variety. Methyl salicylate has a balsamic note from wintergreen oil that is often perceived in aged Verdicchio tasting.

In the Valpolicella area (Verona, Italy) Vitis vinifera cv. Corvina is the main grape variety used to produce Amarone wine. Before starting the winemaking process, the Corvina grapes are stored in a withering (i.e., dehydrating) warehouse until about 30% of the berry weight is lost (WL). This practice is performed to concentrate the metabolites in the berry and enrich the Amarone wine in aroma and antioxidant compounds. In compliance with the guidelines and strict Amarone protocol set by the Consorzio of Amarone Valpolicella, withering must be carried out by setting the grapes in a suitable environment, either under controlled relative air humidity (RH) conditions and wind speed (WS)—no temperature modification is to be applied—or, following the traditional methods, in non-controlled environmental conditions. In general, the two processes have different dehydration kinetics due to the different conditions in terms of temperature, RH, and WS, which affect the accumulation of sugars and organic acids and the biosynthesis of secondary metabolites such as stilbenes and glycoside aroma precursors. For this study, the two grape-withering processes were carried out under controlled (C) and non-controlled (NC) conditions, and the final compositions of the Corvina dried grapes were compared also to evaluate the effects on the organoleptic characteristics of Amarone wine. The findings highlighted differences between the two processes mainly in terms of the secondary metabolites of the dried grapes, which affect the organoleptic characteristics of Amarone wine. Indeed, by the sensory evaluation, wines produced by adopting the NC process were found more harmonious, elegant, and balanced. Finally, we can state how using a traditional system, grapes were characterised by higher levels of VOCs (volatile compounds), whilst wines had a higher and appreciable complexity and finesse.

In the Valpolicella area (Verona, Italy) one of the most important Italian wines is being produced: Amarone, predominately made by Vitis vinifera cv. Corvina. This wine is subjected to a specific postharvest process, namely, withering, which aims to increase alcohol content and/or high residual sugars while retaining richness in aromas and organoleptic compounds. In accordance with guidelines and strict Amarone protocol set by the Consorzio of Amarone-Valpolicella, withering must be carried out by setting the grapes in a suitable environment. In our study, the withering process was performed following the traditional methods, i.e., in open-air, natural environmental conditions, as generally performed by winegrowers, leading to a weight loss in grapes of up to 30%. Whilst the effect of different postharvest conditions is clear and studied, it is interesting to know how soil can affect both grapes and wine profile, in terms of aromas, which are of great importance for this crafted wine. For this purpose, for two study years, the influence of two different sites (with regards to the carbonates’ content) on aromas were investigated. Furthermore, microvinifications and the sensory profile of the resulting wines were analysed. Our results clearly indicated that different soils’ characteristics, particularly differences in carbonate content, had an important effect, not only on the aromatic compounds of grapes and wines, but on the sensory profile as well. This information will help winegrowers and winemakers in the process of determining site selection for future vineyards in order to obtain a final optimum Amarone wine, in terms of its aromatic composition—one that is able to respond to the market demand.

In the Valpolicella area (Verona - Italy) Vitis vinifera cv. Corvina is the main grape variety used to produce Amarone wine. Before starting the winemaking process, the Corvina grapes are stored in a withering (i.e., dehydrating) warehouse until about 30% of the berry weight is lost (WL). This practice is followed to have the chemical metabolites concentrate in the berry and enrich the Amarone wine in aroma and antioxidant compounds. In compliance with the guidelines and strict Amarone protocol set by the Consorzio of Amarone-Valpolicella, withering must be carried out by setting the grapes in a suitable environment, either under controlled relative air humidity (RH) conditions and wind speed (WS) – no temperature modification is to be applied – or, following the traditional methods, in open-air natural environmental conditions. In general, the two processes have different dehydration kinetics due to the different conditions in terms of temperature, RH, and WS, which affect the accumulation of sugars and organic acids and the biosynthesis of sec-ondary metabolites such as stilbenes and glycoside aroma precursors. For this study, the two grape-withering processes were carried out under controlled (C) and not-controlled (NC) condi-tions and the final compositions of the Corvina dried grapes were compared also to evaluate the effects on the organoleptic characteristics of Amarone wine. The findings highlighted differences between the two processes mainly in terms of the secondary metabolites of the dried grapes, which affect the organoleptic characteristics of Amarone wine.

Leaf removal is a viticultural practice applied in order to improve fruit-zone microclimate and berry quality. The aim of this trial was to evaluate the effect of post-flowering leaf removal on maturation and biosynthesis of terpenes and and C13-norisoprenoids in Ribolla Gialla grapes. In the seasons 2015 and 2016, basic maturation parameters were nearly unaffected by leaf removal. Contrarily, in the second season, 2016, one week before harvest, the concentration of several aroma compounds was significantly improved by leaf removal. In conclusion, the trial here showed that leaf removal improves the concentration of aroma compounds in the grapes, but the selection of the date of harvest is more crucial in order to maintain them during vinification.