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The occurrence of knocking combustion is limiting the efficiency of modern spark ignition engine operation. Thus, an understanding of the processes at the knock limit is required for further optimization of the combustion process. In this work, the combustion of a multicomponent Toluene Reference Fuel (TRF) in a single-cylinder research engine is investigated under knocking conditions. The fuel exhibits a negative temperature coefficient (NTC) regime for thermodynamic conditions relevant to the engine operation. A precursor model is used to capture the auto-ignition process. Under homogeneous conditions, a two-stage auto-ignition is observed. Inside the NTC regime, the temperature affects both first-stage and second-stage auto-ignition delay times. With a subsequently conducted multi-cycle engine LES, the effects of temperature stratification and turbulent flame propagation on the local auto-ignition process are investigated. It is observed, that the NTC behavior leads to a widespread two-stage auto-ignition. The knock intensity observed in the experiments is directly related to the mass consumed by auto-ignition. This is due to the fast consumption of the auto-ignited mass by the flame front. With that, the NTC behavior affects the local auto-ignition process in the unburned mixture while the flame propagation determines the knock intensity for the operating conditions at the knock limit.

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