Summary 1.The need for sensitive biological measures of aquatic ecosystem integrity applicable at large spatial scales has been highlighted by the implementation of the European Water Framework Directive. Using fish communities as indicators of habitat quality in rivers, we developed a multi-metric index to test our capacity to (i) correctly model a variety of metrics based on assemblage structure and functions, and (ii) discriminate between the effects of natural vs. human-induced environmental variability at a continental scale. 2. Information was collected for 5252 sites distributed among 1843 European rivers. Data included variables on fish assemblage structure, local environmental variables, sampling strategy and a river basin classification based on native fish fauna similarities accounting for regional effects on local assemblage structure. Fifty-eight metrics reflecting different aspects of fish assemblage structure and function were selected from the available literature and tested for their potential to indicate habitat degradation. 3. To quantify possible deviation from a 'reference condition' for any given site, we first established and validated statistical models describing metric responses to natural environmental variability in the absence of any significant human disturbance. We considered that the residual distributions of these models described the response range of each metric, whatever the natural environmental variability. After testing the sensitivity of these residuals to a gradient of human disturbance, we finally selected 10 metrics that were combined to obtain a European fish assemblage index. We demonstrated that (i) when considering only minimally disturbed sites the index remains invariant, regardless of environmental variability, and (ii) the index shows a significant negative linear response to a gradient of human disturbance. 4. Synthesis and applications . In this reference condition modelling approach, by including a more complete description of environmental variability at both local and regional scales it was possible to develop a novel fish biotic index transferable between catchments at the European scale. The use of functional metrics based on biological attributes of species instead of metrics based on species themselves reduced the index sensitivity to the variability of fish fauna across different biogeographical areas.

Climate change asks for the reduction in the consumption of fossil-based fuels and an increased share of non-regulated renewable energy sources, such as solar and wind power. In order to back up a larger share of these intermittent sources, 'battery services' are needed, currently provided only in large scale by hydropower, leading to more rapid and frequent changes in flows (hydropeaking) in the downstream rivers. Increased knowledge about the ecosystem response to such operations and design of cost-effective measures is needed.We analysed the response of fish communities to hydropeaking (frequency, magnitude, ramping rate and timing) and the interaction with the habitat conditions in Austrian rivers. An index of biotic integrity (Fish Index Austria) was used to compare river sections with varying degrees of flow fluctuations under near-natural and channelized habitat conditions. The results showed that habitat conditions, peak frequency (number of peaks per year), ramping rate (water level variation) and interaction between habitat and ramping rate explained most of the variation of the Fish Index Austria. In addition, peaking during the night seems to harm fish more than peaking during the day. Fish communities in hyporhithral and epipotamal types of rivers are more affected by hydropeaking than those in metarhithral type of rivers. The results support the findings of other studies that fish stranding caused by ramping rates >15 cm h À1 are likely to be the main cause of fish community degradation when occurring more often than 20 times a year. While the ecological status degrades with increasing ramping rate in nature-like rivers, fish communities are heavily degraded in channelized rivers regardless of the ramping rate. The mitigation of hydropeaking, therefore, requires an integrative approach considering the combined effects of hydrological and morphological alterations on fish.

Running water ecosystems of Europe are affected by various human pressures. However, little is known about the prevalence, spatial patterns, interactions with natural environment and co-occurrence of pressures. This study represents the first high-resolution data analysis of human pressures at the European scale, where important pressure criteria for 9330 sampling sites in 14 European countries were analysed. We identified 15 criteria describing major anthropogenic degradation and combined these into a global pressure index by taking additive effects of multiple pressures into account. Rivers are affected by alterations of water quality (59%), hydrology (41%) and morphology (38%). Connectivity is disrupted at the catchment level in 85% and 35% at the river segment level. Approximately 31% of all sites are affected by one, 29% by two, 28% by three and 12% by four pressure groups; only 21% are unaffected. In total, 47% of the sites are multi-impacted. Approximately 90% of lowland rivers are impacted by a combination of all four pressure groups.

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