We
propose monitoring of diabetes through continuous analysis of
undiluted sweat immediately after its excretion using a flow-through
glucose biosensor. The used biosensors are based on Prussian Blue
and glucose oxidase immobilized in perfluorosulfonated ionomer or
gel of alkoxysilane; the resulting sensitivity with the latter reaches
in batch mode 0.23 A M–1 cm–2,
and the calibration range is from 1 μM to 1 mM (flow-through
mode). On the basis of the glucose tolerance test known to be a clinically
relevant procedure to mimic hyperglycemia, a positive correlation
between the rates of glucose concentration increase in blood and in
noninvasively collected sweat has been observed (r = 0.75). The observed correlation between sweat and blood considering
low-molecular weight metabolites is even better than that observed
previously between capillary and vein blood, confirming diagnostic
value of sweat for diabetes monitoring. The dynamics of sweat glucose
concentration, recorded by means of the proposed biosensor, is in
a good accordance with the dynamics of blood glucose content without
any time delay, thus offering a prospect for noninvasive monitoring
of diabetes.
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