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Bartonella infections were investigated in seven species of bats from four regions of the Republic of Georgia. Of the 236 bats that were captured, 212 (90%) specimens were tested for Bartonella infection. Colonies identified as Bartonella were isolated from 105 (49.5%) of 212 bats Phylogenetic analysis based on sequence variation of the gltA gene differentiated 22 unique Bartonella genogroups. Genetic distances between these diverse genogroups were at the level of those observed between different Bartonella species described previously. Twenty-one reference strains from 19 representative genogroups were characterized using four additional genetic markers. Host specificity to bat genera or families was reported for several Bartonella genogroups. Some Bartonella genotypes found in bats clustered with those identified in dogs from Thailand and humans from Poland.

Bats are important reservoirs for many zoonotic pathogens. However, no surveys of bacterial pathogens in bats have been performed in the Caucasus region. To understand the occurrence and distribution of bacterial infections in these mammals, 218 bats belonging to eight species collected from four regions of Georgia were examined for Bartonella, Brucella, Leptospira, and Yersinia using molecular approaches. Bartonella DNA was detected in 77 (35%) bats from all eight species and was distributed in all four regions. The prevalence ranged 6–50% per bat species. The Bartonella DNA represented 25 unique genetic variants that clustered into 21 lineages. Brucella DNA was detected in two Miniopterus schreibersii bats and in two Myotis blythii bats, all of which were from Imereti (west-central region). Leptospira DNA was detected in 25 (13%) bats that included four M. schreibersii bats and 21 M. blythii bats collected from two regions. The Leptospira sequences represented five genetic variants with one of them being closely related to the zoonotic pathogen L. interrogans (98.6% genetic identity). No Yersinia DNA was detected in the bats. Mixed infections were observed in several cases. One M. blythii bat and one M. schreibersii bat were co-infected with Bartonella, Brucella, and Leptospira; one M. blythii bat and one M. schreibersii bat were co-infected with Bartonella and Brucella; 15 M. blythii bats and three M. schreibersii bats were co-infected with Bartonella and Leptospira. Our results suggest that bats in Georgia are exposed to multiple bacterial infections. Further studies are needed to evaluate pathogenicity of these agents to bats and their zoonotic potential.

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Of the 108 species that occur in Georgia, ten species are insectivores belonging to the order Eulipotyphla. Forty percent of them are endemic to the Caucasus and sixty percent are endemic to the Middle East, including the Caucasus. Up to now, no comprehensive data on the distribution of insectivores in Georgia have been available. The aggregated standardised data on the occurrence of small mammals can be applied to resource management, biogeography, ecological and systematic studies and to the planning of nature conservation efforts. Hereafter, the attempt to provide accumulated in one paper all known points of insectivores' occurrence in Georgia and make it available to researchers via the open repository GBIF is presented. The dataset is based on both literature data from 30 published sources (251 records), collection vouchers from four main zoological collections containing vouchers from Georgia (415 records) and authors' fieldwork results (217 records). The occurrence points of the specimens stored in collections and museums have been extracted from museum voucher labels and museum journals. All known sampling points of insectivores in Georgia are collected in one dataset for the first time. Our field surveillance data reach about 24.6% of the records. Most of our data collected since 2003 have not been published yet. About 28.4% of the records have been recovered from publications in Russian and Georgian languages and 47% of the dataset records are derived from collections.