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In this work, a novel nanobiocatalyst (L–Mcn4@Ca2Fe2O5) was synthesized from Bacillus lipase on srebrodolskite nanoparticles. Bacillus sp. MCn4 culture media was optimized reaching the maximum lipolytic activity using peptone 7.5 g/L and sucrose 5 g/L and Ca2Fe2O5, was synthesized and characterized (size of 100–150 nm). L–Mcn4@Ca2Fe2O5 was obtained by adsorption (12 U/mg oxide) and successfully characterized by different techniques. According to the best immobilization conditions, pH 4 and 100 Mm ionic strength, an adsorption model with two stages was proposed: initial interfacial activation followed by multi‐point electrostatic interactions. Stability studies showed an enhancement in its performance between 50 and 70 °C and against ethanol compared with free enzyme and the thermal decomposition process was softer when lipase was immobilized. The biocatalyst hydrolyzed fish oil showing enrichment in the PUFAs content with an increase on DHA of 1.2 fold showing a great potential for its use in the food industry.

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