ChAMP is implemented as a Bioconductor package in R. The package and the vignette can be downloaded at bioconductor.org

Data Availability Sequence data used during the study will be deposited at the European Genome-phenome Archive (EGA), which is hosted by The European Bioinformatics Institute (EBI) and the Centre for Genomic Regulation (CRG) under the accession code: EGAS00001003458. Further information about EGA can be found at https://ega-archive.org.

SummaryThe Illumina Infinium HumanMethylationEPIC BeadChip is the new platform for high-throughput DNA methylation analysis, effectively doubling the coverage compared to the older 450 K array. Here we present a significantly updated and improved version of the Bioconductor package ChAMP, which can be used to analyze EPIC and 450k data. Many enhanced functionalities have been added, including correction for cell-type heterogeneity, network analysis and a series of interactive graphical user interfaces.Availability and implementation ChAMP is a BioC package available from https://bioconductor.org/packages/release/bioc/html/ChAMP.html.Supplementary information Supplementary data are available at Bioinformatics online.

Expression profiles of miRNA distinguish esophageal tumor histology and can discriminate normal tissue from tumor. MicroRNA expression may prove useful for identifying patients with Barrett esophagus at high risk for progression to adenocarcinoma.