Many agricultural producers apply glyphosate to glyphosate-resistant crops to control weeds, including Palmer amaranth. Populations of this weed in Tennessee not completely controlled by glyphosate were examined. Field and greenhouse research confirmed that two separate populations had reduced biomass sensitivity (1.5× to 5.0×) to glyphosate compared to susceptible populations, although the level of resistance was higher based on plant mortality response (about 10×). Shikimate accumulated in both resistant and susceptible plants, indicating that 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was inhibited in both biotypes. These results suggest that an altered target site is not responsible for glyphosate resistance in these Palmer amaranth biotypes.

Preplant applications of two 2,4-D formulations and dicamba were evaluated for injury to no-till soybean. Herbicides applied 28 or 21 d before planting (DBP) at 0.56 kg ae/ha for the 2,4-D formulations and 0.28 kg ae/ha for dicamba did not injure soybean. At 14 DBP soybean injury was lower (< 6%) with applications of 2,4-D than with dicamba (13% to 17%). Injury increased when herbicides were applied at 7 DBP with soybean injury from both 2,4-D (< 11%) and dicamba (< 38%). When the herbicides were applied at planting soybean injury was < 18% with the 2,4-D formulations and < 73% with dicamba. Soybean yields were unaffected by the injury from herbicides applied 21, 14, and 7 DBP. However, soybean yield was decreased to 870 kg/ha in 2005 when dicamba was applied at planting compared to the nontreated check (1,950 kg/ha)

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