Background: Sepsis is a serious health problem and is associated with life-threatening complications, such as liver dysfunction, renal failure as well as cardiorespiratory disease. Kupffer cells are immune cells that contribute to the pathogenesis of liver dysfunction. This study aims to determine the effect of ketamine on the number of Kupffer cells in experimental animal models of sepsis. Methodology: This experimental study used fecal-induced peritonitis (FIP) to create a sepsis model in rats (Rattus norvegicus). Thirty rats were divided into six equal groups, namely the negative control group, which was not treated with FIP (Group K−), the positive control group or the sepsis model with FIP treatment (Group K+), and the treatment group; the sepsis model rats (FIP treatment) given 5 mg of ketamine/kg intraperitoneally once at 0 h (Group A), at 3 h (Group B), 5 h (Group C) after induction of sepsis with FIP, and every 2 h after induction of sepsis with FIP for four hours (Group D). Kupffer cells were counted six hours after FIP induction using a hematology analyzer. Statistical test was carried out using the One-Way ANOVA test using SPSS 18.0 software. Results: The number of Kupffer cells in sepsis groups (K+) was significantly higher (22.60 cell/ml) than in the negative control group (K-) (12.68 cell/ml) (P= 0.01). The administration of ketamine in the sepsis model group significantly decreased the number of Kupffer cells close to normal. The number of Kupffer cells was 12.56 cells/ml in Group A, 12.92 cells/ml in Group B, 9.75 cells/ml in Group C, and 8.50 cells/ml in Group D. Conclusion: The administration of ketamine decreased the number of Kupffer cells in the rat model of sepsis induced by FIP to close to the normal group. Abbreviations:  APP - acute-phase protein; FIP - fecal induced peritonitis; ICU - Intensive Care Unit; IL – interleukin; KCs - Kupffer cells; ROS - reactive oxygen species; NO - nitric oxide; SIRS - systemic inflammatory response syndrome; TNF-α  - tumor necrosis factor-alpha. Keywords: Immunomodulator; Ketamine; Kupffer cell; Sepsis Citation: Asmoro AA, Jaya W, Wahyudi AN, Laksono RM. The effect of ketamine on Kupffer cell count in Wistar rat (Rattus norvegicus) model of sepsis. Anaesth. pain intensive care 2022;26(4): 445-449. DOI: 10.35975/apic.v26i4.1946 Received: April 20, 2022; Reviewed: July 13, 2022; Accepted: July 13, 2022