Terpenoid compound from ara's leaf is known to have immunosuppressant activity by inhibit blood cells proliferation. Immunosuppressant is a group of drugs that can suppress excessive immune responses. The aim of this research was investigated the immunosuppressant eff ect of petroleum ether from ara's leaf (Ficus racemosa Linn.) on mice (Mus musculus). The immunosuppressant activity was conducted by carbon clearance method to investigate the ability of phagocytosis with carbon as an antigen that is injected intravenously, followed by determining organ index. All data was analyzed by SPSS. The result showed that at dose of 560 mg/kgbw and 1120 mg/kgbw, extract found to have immunostimulant activity with phagocytic index values respectively of 1.5 and 1.1, while the dose of 1680 mg/kgbw was immunosuppressant with phagocytic index 0.7. The lymphoid organ index showed a signifi cantly diff erence between petroleum ether from ara leaf extract by dose 1680 mg/kgbw with negative control (p<0.05). Based on the results of the non-specifi c immune response, it can be concluded that the petroleum ether ara's leaf extract by dose 1680 mg/kgbw has immunosuppressant activity.Abstrak: Senyawa terpenoid daun ara diketahui memiliki aktivitas imunosupresan dengan menghambat proliferasi sel darah. Imunosupresan merupakan golongan obat yang dapat menekan respon imun berlebihan. Penelitian ini bertujuan untuk mengetahui aktivitas imunosupresan ekstrak petroleum eter daun ara (Ficus racemosa. Linn) terhadap mencit (Muss musculus). Uji imunosupresan dilakukan dengan metode bersihan karbon untuk mengetahui kemampuan fagositosis dengan karbon sebagai antigen yang diinjeksikan intravena, dilanjutkan dengan menghitung indeks organ. Seluruh data yang diperoleh kemudian dianalisis statistik menggunakan SPSS. Hasil uji bersihan karbon menunjukkan bahwa ekstrak petroleum eter daun ara dosis 560 mg/kgBB bersifat imunostimulan sedang dosis 1120 mg/kgBB bersifat imunostimulan lemah dengan nilai indeks fagositosis berturut-turut 1,5 dan 1,1; sedangkan dosis 1680 mg/kgBB bersifat imunosupresan dengan indeks fagositosis 0,7. Hasil uji indeks organ limfoid menunjukkan perbedaan signifi kan antara ekstrak petroleum eter dosis 1680 mg/kgBB dengan kontrol negatif (p<0,05). Berdasarkan hasil uji respon imun non spesifi k disimpulkan bahwa ekstrak petroleum eter daun ara dosis 1680 mg/kgBB mempunyai aktivitas imunosupresan.Kata kunci: Imunosupresan, Ficus racemosa Linn., fagositosis, bersihan karbon.