Introduction
Oxidative tissue damage caused by reactive oxygen species results in a significant decrease in the total antioxidant capacity of the biological system. The aim of this interdisciplinary study was to answer the question of whether active antioxidants modify, at a molecular and supramolecular level, the tissue of pathological amnion and the necrotic eschar degraded in thermal burn.
Methods
A Nicolet 6700 Fourier-transform spectrophotometer with OMNIC software and the EasiDiff diffusion accessory were used in the FTIR spectroscopic analysis. A NICOLET MAGNA-IR 860 spectrometer with FT-Raman accessory was used to record the Raman spectra of the samples. The samples were exposed to bacteria capable of causing nosocomial infections, ie Gram-positive
Staphylococcus aureus
and Gram-negative
Escherichia coli
and
Pseudomonas aeruginosa
. Whereas samples of hypotrophic amnion interacted with
Staphylococcus aureus, Escherichia coli
and
Enterococcus faecalis
. The obtained flame retardant effect of placentas was evaluated using the method of the limiting oxygen index (LOI).
Results
The infrared spectroscopy analysis proved that after modification of the amniotic samples in graphene oxide and ortho-silicic acid, the amide II band is split into two components. Incubation of samples in modifier solutions: graphene oxide, sodium ascorbate and L-ascorbic acid results in shifts and changes of intensity within the broadly understood lipid band 1743–1745–1747 cm
−1
. The oxidising changes observed within the lipid and amide bands are affected by the incubation effect of graphene oxide as a modifier, possibly adsorbing on the surface of the amniotic membrane. On the basis of microbiological studies, pathogenic bacteria commonly causing amniotic infections and growing in burn wounds were found to have particularly good resistance to stabilized ortho-silicic acid (
E. coli
) and lactoferrin (
S.aureus
).
Conclusion
This thermogravimetric study found the highest stability of the analysed tissues (hypotrophic amnion and burnt epidermis) after modification with graphene oxide and sodium ascorbate.
Innovative geotextiles built from meandrically arranged thick ropes were produced. For the production of the geotextiles, the strips of woollen nonwoven were used. The segments of the geotextiles were installed on the bank of a drainage ditch. The behaviour of the geotextiles during 1 year was observed. It was demonstrated that the geotextiles installed in the ditch provide immediate protection of the bank. In the soil-covered wool, the process of slow biodegradation was initiated. Because of a low biodegradation rate at the end of the growing season, when the protective vegetation was not well developed, the geotextiles maintained their protective potential. In the following months, the biodegradation led to further destruction of wool fibres. During the biodegradation, the organic compounds rich in nitrogen were released into the soil. In spring, at the beginning of new growing season, the compounds acted as effective fertilizers, promoting the growth of protective vegetation. The vegetation growing on the bank provided an effective protection and took over the protective function of the geotextiles.
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