Objective: In this study, the effects of a green banana pasta diet on the oxidative damage from type 1 diabetes mellitus (DM) were investigated. Materials and methods: Formulations containing 25 (F25), 50 (F50), and 75% (F75) of green banana pasta were prepared and included in a 12-week diet of Wistar rats with alloxan-induced type 1 DM. The effects of these formulations in preventing oxidative damage in kidneys and liver homogenates of rats were evaluated using the TBARS assay (lipid peroxidation in liver) and the DNPH assay (protein oxidation in liver and kidneys). Furthermore, the effects of the formulations on the fasting glycemia, fructosamine levels, renal function (creatinine), liver function (enzymes aspartate aminotransferase [AST] and alanine aminotransferase [ALT]), and lipid profile (total cholesterol and fractions) in the serum of rats were evaluated in addition to the evaluation of the centesimal composition and microbiological analysis of the produced green banana pasta. Results: An F75 diet prevented hyperglycemia in diabetic rats (p < 0.05) compared to the diabetic rats fed a standard diet (commercial feed). Notably, the protein oxidation in both the liver and kidneys were prevented in diabetic rats on the F50 or F75 diets compared to the control group, whereas the lipid peroxidation was only prevented in the liver (p < 0.05). Moreover, all formulations prevented an increase in the amount of triglycerides in the serum of the rats. The F25 and F50 diet prevented the increase of cholesterol, and the F75-based diet of ALT and fructosamine (p < 0.05) supported the anti-hyperglycemic effects and the protection against oxidative damage. Conclusion: The green banana pasta (F75) diet showed great potential for preventing complications associated with diabetes. Arch Endocrinol Metab. 2016;60(4):355-66

Introduction: Diabetes mellitus (DM) is still considered a major public health concern worldwide and lacks new and safe drugs for long-term and/or adjuvant therapy. Thus, we postulated that nitroxides could prevent DM complications in rats. Methods: To evaluate the effects of the nitroxides tempo and tempol in preventing hyperglycemia, heart damage, DM-associated oxidative stress-induced renal injury, and other DM complications, nitroxides (30 mg/kg/day) were fed to Wistar rats with alloxan-induced type 1 DM for 60 days. The animals were then evaluated for glucose levels, lipid peroxidation in the kidneys, biochemical parameters of renal function (creatinine levels), liver function (aspartate aminotransferase [AST] and alanine aminotransferase [ALT] total lipids, and steatosis), and lipid profi ling (total cholesterol and fractions), and a histological analysis of the kidneys, liver, heart, and eyes was performed. Results: Treatment of the diabetic rats with tempo or empol prevented hyperglycemia (p < 0.01). Interestingly, only tempo was able to prevent lipid peroxidation in the kidneys of the diabetic rats (p < 0.01), while also decreasing creatinine levels (p < 0.05). In contrast, tempol prevented damage in the liver, kidneys, eyes, and heart of diabetic rats (p < 0.01) and showed evidence of preventing left ventricular hypertrophy. Notably, tempol and tempo prevented any signifi cant increase in ALT levels and in the amount of total lipids in the liver of the diabetic rats. Conclusion: tempo and tempol presented great potential for preventing hyperglycemia and its complications in rats, both of which decreased markers of liver dysfunction. Tempo also mitigated oxidative damage to the kidneys, while tempol considerably attenuated heart injury. Nitróxidos Melhoram os Parâmetros Bioquímicos e Previnem ILesões em Orgãos de Ratos com Diabetes Mellitus Tipo 1 Introdução: A diabetes mellitus (DM) ainda é considerada uma das principais preocupações de saúde pública em todo o mundo e carece de medicamentos novos e seguros para uma terapia de longo prazo e/ou adjuvante. Assim, postulamos que os nitróxidos poderiam prevenir as complicações do DM em ratos. Métodos: Para avaliar os efeitos dos nitróxidos tempo e tempol na prevenção de hiperglicemia, lesão cardíaca, lesão renal induzida por estresse oxidativo e outras complicações do DM, os nitróxidos (30 mg/kg/dia) foram administrados durante 60 dias a ratos Wistar com DM tipo 1 induzido por Historial do artigo: