The inhibiting activity of three yeast strains belonging to Pichia kudriavzevii, Pichia occidentalis, and Meyerozyma quilliermondii/Meyerozyma caribbica genera against common plant pathogens representing Mucor spp., Penicillium chrysogenum, Penicillium expansum, Aspergillus flavus, Fusarium cereals, Fusarium poae, as well as Botrytis cinerea genera was investigated. The yeast strains tested had a positive impact on growth inhibition of all target plant pathogens. The degree of inhibition was more than 50% and varied depending on both the yeast antagonist and the mold. Ethyl esters of medium-chain fatty acids, phenylethyl alcohol, and its acetate ester prevailed among the analyzed volatile organic compounds (VOCs) emitted by yeasts in the presence of the target plant pathogens. Due to the method used, assuming no contact between the antagonist and the pathogen, the antagonistic activity of the yeast strains studied resulted mainly from the production of biologically active VOCs. Moreover, the antagonistic activity was not only restricted to a single plant pathogen but effective towards molds of different genera, making the yeast strains studied very useful for potential application in biological control.

The aim of this study was to investigate the association of the g.710T>G polymorphism in the keratin gene, which results in a cysteine to glycine amino acid change at position 83 (Cys83Gly) in feather keratin, with homing pigeon racing performance. A total of 123 homing pigeons were investigated. The data set used in this study consisted of scores from 17 short races (less than 400 km) and 11 long races (greater than 500 km) that took place in the 2011 and 2012 racing seasons (2589 race records in total). The genotyping of the g.710T>G polymorphism was performed using the artificially created restriction site-PCR assay. The T allele and the TT genotype were prevalent with frequencies of 0.658 and 0.447, respectively. The TT pigeons had the highest mean of ace points in the long races and in all races overall, while the GT birds scored the best in the short races. Nevertheless, the effect of the polymorphism was significant only in the long races (P = 0.0451), in which the pigeons carrying the TT genotype showed better racing performance in comparison with those carrying the GG genotype (P ≤ 0.05). In order to explain this phenomenon, several bioinformatics tools were employed to check for the possible consequences of the Cys83Gly substitution for feather keratin. The cysteine at position 83 was indicated to form a disulphide bond, while the Cys83Gly substitution was predicted to disturb the stability of the protein. However, the predictions preformed using the different tools were not entirely consistent. Nevertheless, the loss of the cysteine at position 83 of pigeon feather keratin may affect the structure of feathers, thus changing their biomechanical characteristics, and consequently, may influence the flying ability of pigeons.

The T > C single nucleotide polymorphism (SNP) in the MIR2467 gene was investigated in order to confirm its presence in cattle genome and to check for possible differences in its genotype distribution among different breeds. Additional purpose of the study was to investigate in silico potential effect of that substitution on the structure and stability of precursor mir-2467. The study involved 634 individuals of five cattle breeds: Angus, Hereford, Holstein-Friesian, Jersey, and Limousin, which were genotyped using PCR-RFLP assay. In this study, the presence of T > C polymorphism at position 24 was observed in all the cattle breeds excepting Hereford. In addition, the differences in the genotype distribution among analyzed breeds were indicated. On the basis of minimum free energy structure prediction, the C allele was indicated to have possible impact on decreasing the stability of the pre-mir-2467, thus altering its ability to regulate target genes expression.