The process of oxidation, due to free radicals, is the cause of major concern for human health. In particular damages related to the skin have great relevance; therefore, many antioxidants based products are developed and marketed with the intention to counteract the action of free radicals. The seed of Araucaria angustifolia is a rich source of antioxidants due to the presence of bioflavonoids to counteract free radicals damages. In this study, two extracts, one from the seed external teguments (shell) and the other from the inner seed pulp (endosperm and embryo) were obtained in order to evaluate possible applications to the dermo-cosmetic field. In parallel the following different methods were employed to characterize both the extracts and to determine their antioxidant capacity: HPTLC, ABTS and DPPH, ORAC and PLC. The qualitative analysis showed that both extracts have the antioxidant activity, but the quantitative evaluation revealed a more promising bioactivity from the shell than from the pulp. Therefore, it was evaluated the potential skin application of different cosmetic formulations, based on the presence of seed shell extract (W/O emulsion, O/W emulsion and gel). The best result was achieved with the W/O emulsion.

BACKGROUND The use of bioreactors that mimic industrial reality is essential if knowledge transfer is desired. This is particularly important once filamentous microbes face harsh conditions hindering the formation of biofilms in some types of bioreactors and thus diminishing their ability to perform biotransformation or production of secondary metabolites. Airlift bioreactors can circumvent such problems; however, the lack of consensus regarding riser and downcomer partition persists. The use of golden ratio is a possibility to be considered. Our proposition was to evaluate the hydrodynamic performance and mass transfer in split‐rectangle‐internal loop airlift bioreactors (SRILAB) using a golden ratio partition. RESULTS In a general way, results revealed that a partition of Ar/Ad1.618 presents better and more desirable characteristics than Ad/Ar1.618 for linear liquid velocity (UL), mixing time (tM), gas holdup (ϵG), and volumetric oxygen transfer coefficient (kLa). Such behavior was observed even when the SRILAB operated under different linear gas velocities (UG) regimens (0.5–1.5 vvm) and Newtonian and non‐Newtonian bulk fluids. CONCLUSION Such results support the idea of using the golden ratio as riser‐to‐downcomer partitioning parameter in airlift bioreactors. © 2018 Society of Chemical Industry

Dysbiosis is characterized by a disruption of bacterial homeostasis and may be associated with various skin diseases. Acne is a multifactorial inflammatory disease with a robust microbial component and numerous correlations with dysbiosis states. Furthermore, various factors are recognized as triggers for skin dysbiosis, including the use of certain cosmetics. Based on these arguments, we hypothesized that the use of photoprotective formulations could trigger dysbiosis and the occurrence of acne manifestations. To verify this assumption, six volunteers between 19 and 23 years of age, meeting all the inclusion criteria, received two applications a day of a non-commercial sunscreen formulation developed with the sun filters ethylhexyl methoxycinnamate, ethylhexyl salicylate, methyl anthranilate, and octocrylene dispersed in a base gel, with an estimated protection factor of 28.8. The pure base gel was used as a control. The samples were applied to an area delimited by a standard template (15 cm2) in an amount corresponding to 30 mg (2 mg cm2) for ten days. At two points in time, pre- and post-sample applications, the facial skin surface was swabbed to collect extracted DNA and processed to verify divergent degrees of 16S RNA coding sequences. The data obtained allowed us to determine the abundance of different bacterial entities at the genus and species levels. The results showed that key species of the acne process, such as Cutibacterium acnes and Staphylococcus epidermidis, seem to tolerate the evaluated formulation well, not being significantly affected by the formulation, suggesting no interference of its use concerning dysbiosis induction.

Um dos maiores problemas a ser resolvido em serviços de saúde é o fato de que muitos micro-organismospatogênicos podem crescer sobre superfícies sob o fenótipo de biofi lmes. Células microbianas crescidas sob ofenótipo de biofi lmes normalmente se encontram imobilizadas na matriz polimérica extracelular e apresentamforam desenvolvidas três formulações detergentes para uso em ambiente clínico-hospitalar-odontológico,contendo digluconato de clorexidina 2% em bases catiônica, aniônica e não iônica. Essas formulações foramtestadas quanto às suas capacidades de inativação de biofi lmes de Staphylococcus aureus ATCC®25933®, Pseudomonasaeruginosa ATCC®27853® e Candida albicans SC5314. Foi mensurada a ação detergente das três formulaçõessobre os biofi lmes de 24h das três entidades microbianas e foi empregado o ensaio de retenção de cristalvioleta 0,5%. De forma surpreendente, na formulação com base aniônica, deforma inversa ao esperado, aclorexidina comprometeu parcialmente a atividade inativante do detergente, talvez por precipitação do tensioativo.Os resultados mostraram que os componentes das formulações detergentes aqui testadas parecemser mais determinantes na inativação das células microbianas dos biofi lmes que a clorexidina.

Considerando como objetivo o desenvolvimento de competências como autonomia, habilidade na resolução de problemas, inovação e senso crítico, os métodos de ensino tradicionais foram substituídos por metodologias inovadoras, proporcionando melhores condições para o desenvolvimento das competências, de forma mais atraente e efetiva. Entre as novas ferramentas sugeridas  para a condução do ensino sob medida  podemos citar com destaque a relevância  da avaliação diagnóstica que permite  um melhor planejamento das estratégias de  ensino aprendizagem. Sobre o presente estudo conduzido com estudantes da disciplina de tecnologia de cosméticos do curso de farmácia, a avaliação diagnóstica mostrou-se fundamental na percepção de deficiências  no trajeto pregresso do estudante, possibilitando ajustes estratégicos no desenvolvimento dos trabalhos propostos.