Introduction: Quality of life (QOL) may refer to a conceptual and quite complex discussion due to specific definition lack and poor procedures and instruments able to measure it. In this study, QOL’s instrument validation is based upon stressor and stress triggering agents. Foreign instruments may not always reach their goals as Brazilian and Latin American cultural differences shall compromise the data quality. In Brazil, questionnaires assessing organizational environment stress are scarce and often not validated. Objective: to demonstrate the construction and validation of an easy to apply instrument gathering stress and QOL. As a question problem, it was asked: What steps are to be followed for the construction and validation of an instrument? Methods: A 25 questions instrument was divided into four areas: Work environment; social relations; leisure; sleep. Six steps for instrument validation were followed: 1- Content Validity (or construct); 2- Internal Consistency; 3- Discriminant Validity; 4- Criterion Validity; 5- Concurrent Validity; and 6- Temporal Reproducibility. Results: The data is coherent to the instrument initially thought. Conclusions: The instrument is valid and reliable. It may be concluded that it effectively measures what it proposes to, consistently demonstrating success