Introduction: Inborn errors of metabolism (IEM) represent an important public health problem due to current diagnosis and treatment limitations, poor life quality of affected patients, and consequent untimely child death. In contrast to classical methods, tandem mass spectrometry (MS/MS) has allowed simultaneous evaluation of multiple metabolites associated with IEM offering higher sensitivity, low false positive rates and high throughput.Aims: Determine concentration levels for amino acids and acylcarnitines in blood of newborns from Colombia, to establish reference values for further use in diagnosis of IEM.Methods: Implementation of a method to determine amino acids, acylcarnitines and succinylacetone in newborn dried blood spots using MS/MS, and its application in a cross-sectional study conducted in 891 healthy neonates from Cali and Quibdo cities is described. Results: fifty-seven analytes that allow the diagnosis of more than 40 different pathologies were tested. The method showed to be linear, precise and accurate. Healthy neonates 1-18 days of age were included, 523 from Cali and 368 from Quibdo; 52% male and 48% female. Age-related differences on the concentration levels of amino acids and acylcarnitines were observed whereas no significant differences by gender were found. Conclusion: The study has contributed to reveal the usual concentration levels of amino acids, acylcarnitines and succinylacetone that could be used as reference for the establishment of a newborn metabolic screening program in Colombia.

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Catalyst-Free Synthesis of Pyrazolo[3,4-b]pyrrolo[3,4-d]pyridine Derivatives (III) by Aza-Diels-AlderReaction. -(QUIROGA*, J.; VALENCIA, A.; PEREZ, A.; GALVEZ, J.; ABONIA, R.; INSUASTY, B.; Lett. Org. Chem. 10 (2013) 5, 337-341, http://dx.

Catalyst Free Three-Component Synthesis of (±)-Pyrazolylpyrrolopyrroles by 1,3-Dipolar Cycloaddition Reaction. -A simple one-pot procedure allows the synthesis of title compounds (IV) via endo-selective 1,3-dipolar cycloaddition of ylides, generated in situ from aldehydes (I) and esters (II), with 1-arylmaleimides (III). -(QUIROGA*, J.; GALVEZ, J.; PEREZ, A.; VALENCIA, A.; ABONIA, R.; INSUASTY, B.; Tetrahedron Lett. 52 (2011) 42, 5471-5473, http://dx.