This study aimed to analyze the effect of a single bout of high-intensity intermittent exercise (HIIE) on serum adiponectin levels in sedentary overweight young adult women. A total of 22 overweight adult obesity women with a BMI of 23-24.9 m2/kg were enrolled in this study. Subjects were divided into 2 groups, control and HIIE. The exercise was static cycling for a total of 25 minutes (3 minutes warming up, 20 minutes HIIE, and 2 minutes cooling down). The HIIE was held 10 sets intermittently with the ratio 1:1, 1 minute cycling in 80-90% HRmax, 1 minute active resting with 40 rpm cycling. The data in this study were taken before and 1 hour after the HIIE. The adiponectin level was measured using a human ELISA kit. The adiponectin level in the control group reduced significantly (p<0.05), however, the HIIE group reduced insignificantly. A single bout of HIIE in sedentary overweight young adult women can maintain the adiponectin level. Therefore, it may have a positive contribution to women’s health. However, further research needs to be conducted to uncover the underlying mechanism.