El estudio de levaduras No-Saccharomyces (NSY) en fermentaciones enológicas permite explorar nuevas alternativas para la reducción de etanol en vinos. El objetivo de este trabajo fue evaluar la capacidad fermentativa de dos levaduras autóctonas y poco exploradas del tipo NSY (NSYa, NSYb) en fermentaciones monocultivo y secuenciales (escala laboratorio y microvinificación) para producir vino Sauvignon Blanc chileno. Las fermentaciones se monitorearon mediante la determinación de etanol, glicerol, ácidos orgánicos y azúcares residuales. Los resultados indicaron que a escala de laboratorio tanto para las fermentaciones monocultivo y secuenciales fue posible reducir la concentración de etanol, a un 0,77% v/v (monocultivo) y 1,5% v/v (secuencial) para NSYa y 0,50% v/v (monocultivo) y 0,04% v/v (secuencial) para NSYb comparado con S. cerevisiae (12,87% v/v). Adicionalmente, mayores concentraciones de glicerol fueron obtenidas en fermentaciones monocultivo en comparación a las secuenciales (NSYa: 9,47 g/L y NSYb 10,97 g/L). A escala de microvinificación, las fermentaciones monocultivo y secuenciales con NSYb lograron reducir el contenido de etanol en 0,17% v/v y 0,54% v/v, respectivamente, comparado al control de S. cerevisiae (13,74% v/v). En el caso de NSYa, la reducción solo se observó en fermentaciones secuenciales con 0,62% v/v

The seed from the Araucaria araucana (in Spanish, piñon) tree, native to Chile and Argentina, is sold mainly as raw seed. Engineering a process to add value to piñon has the potential to positively impact local indigenous communities with very little ecological impact because it is routinely harvested in the wild. This study evaluated the feasibility of using 100% piñon, or as a blend with barley malt, to produce a beer-like beverage, while also evaluating consumer acceptance of the beverage’s piñon characteristics. Prototypes generated based on 93% piñon and 7% oat (enzymatic treatment of α-amylase, glucoamylase, protease and β-glucanase), as well as 50% piñon and 50% barley (no external enzymatic treatment), were evaluated. Overall acceptability by a consumer acceptance panel (21 consumers) rated the 100% piñon and the piñon–barley malt blend 5/9 and 7/9, respectively. The piñon–barley malt blend prototype stood out for its low level of carbohydrates, high potassium content and banana and clove aromas.

The study of non-Saccharomyces yeasts in wine fermentations allows the exploration of new alternatives for the reduction of ethanol in wines. The objective of this work was to evaluate the fermentation capacity of two indigenous Candida yeasts (C. oleophila and C. boidinii) in monoculture and sequential fermentations (laboratory and microvinification scale) to produce Chilean Sauvignon Blanc wine. Fermentations were monitored by the determination of ethanol, glycerol, organic acids, and residual sugars. The results indicated that at the laboratory scale for both the monoculture and sequential fermentations it was possible to reduce the ethanol concentration on 0.77% v/v (monoculture) and 1.5% v/v (sequential) for C. oleophila and 0.50% v/v (monoculture) and 0.04% v/v (sequential) for C. boidinii compared to S. cerevisiae (12.87% v/v). Higher glycerol concentrations were produced in monoculture than sequential fermentations (C. oleophila: 9.47 g/L and C. boidinii 10.97 g/L). For microvinifications, the monoculture and sequential fermentations with C. boidinii managed to reduce ethanol content by 0.17% v/v and 0.54% v/v, respectively, over the S. cerevisiae control (13.74% v/v). In the case of C. oleophila, the reduction was only observed in sequential fermentations with 0.62% v/v. Interestingly, grapes with higher sugar concentration resulted in wines with lees ethanol concentrations. This might be associated to the use of C. oleophila (13.12% v/v) and C. boidinii (13.20% v/v) in sequential fermentations microvinification scale.