a-Formylphenylacetic Acid, Phenylmalonic Semialdehyde, Tropic Acid Biosynthesis a-Formylphenylacetic acid, the postulated immediate precursor of tropic acid, has been synthesized by deacetalization of a-diethoxymethylphenylacetic acid in the presence of silica gel. The compound was reasonably stable in organic solution. In aqueous media, however, a pronounced lability of this semialdehyde was observed at various pH-values (t/2 = 4.5 min at pH 7.4). It is thus very unlikely that this compound can be employed successfully in biosynthetic studies.
The reaction between prothrombin and staphylocoagulase is studied.a. Optimal amounts of the active reaction product (coagulase-thrombin) are found when equimolar amounts of prothrombin and staphylocoagulase are added together.b. The molecular weight of coagulase-thrombin equals the sum of the molecular weights of staphylocoagulase and prothrombin, both when estimated by gelfiltration and by SDS-polyacrylamide gel electrophoresis.c. The amino acid composition of coagulase-thrombin is not in contrast with the sum of the amino acid compositions of staphylocoagulase and prothrombin.d. In a preparation of coagulase-thrombin the N-terminal amino acids are those of prothrombin (alanine) and staphylocoagulase (aspartic acid).e. An antibody against coagulase-thrombin precipitates both prothrombin and staphylocoagulase but not thrombin.f. It is concluded that the thrombin acitivity in coagulase-thrombin is a result from a stoichiometric reaction between one molecule of prothrombin and one molecule of staphylocoagulase and that limited proteolysis does not play a role in this mechanism.
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