The use of this cell-free collagen type I scaffold for large defects showed increased wear of the repair tissue and clinical failure in 18% of cases at 5-year follow-up.

Lung metastases in healthy patients should be removed non-anatomically whenever possible. This can be done with a laser. Lung parenchyma can be cut very well, because of its high energy absorption at a wavelength of 1940 nm. A coagulation layer is created on the resected surface. It is not clear, whether this surface also needs to be sutured to ensure that it remains airtight even at higher ventilation pressures. It would be helpful, if suturing could be avoided, because the lung can become too puckered, especially with multiple resections, resulting in considerable restriction. We carried out our experiments on isolated and ventilated paracardiac lung lobes of pigs. Non-anatomic resection was carried out reproducibly using three different thulium laser fibres (230, 365 and 600 μm) at two different laser power levels (10 W, 30 W) and three different resection depths (0.5, 1.0 and 2.0 cm). Initial airtightness was investigated while ventilating at normal frequency. We also investigated the bursting pressures of the resected areas by increasing the inspiratory pressure. When 230- and 365-μm fibres were used with a power of 10 W, 70 % of samples were initially airtight up to a resection depth of 1 cm. This rate fell at depths of up to 2 cm. All resected surfaces remained airtight during ventilation when 600-μm fibres were used at both laser power levels (10 and 30 W). The bursting pressures achieved with 600-μm fibres were higher than with the other fibres used: 0.5 cm, 41.6 ± 3.2 mbar; 1 cm, 38.2 ± 2.5 mbar; 2 cm, 33.7 ± 4.8 mbar. As laser power and thickness of laser fibre increased, so the coagulation zone became thicker. With a 600-μm fibre, it measured 145.0 ± 8.2 μm with 10 W power and 315.5 ± 6.4 μm with 30 W power. Closure with sutures after non-anatomic resection of lung parenchyma is not necessary when a thulium laser is used provided a 600-μm fibre and adequate laser power (30 W) are employed. At deeper resection levels, the risk of cutting small segmental bronchi is considerably increased. They must always be closed with sutures.

Background Cerebellar degeneration as a consequence of a malignancy is a rare condition most commonly related to the presence of anti-Yo, anti-Hu, and anti-Tr/DNER antibodies. In recent years, several reports have indicated Zinc-finger protein 4 (Zic4) antibodies being associated with paraneoplastic cerebellar degeneration (PCD) in patients with small cell lung carcinoma. However, the prevalence and the significance of Zic4-antibodies may be underestimated due to their co-occurrence with more frequent antibodies such as anti-Hu. A literature review of isolated Zic4 mediated paraneoplastic syndromes yielded 14 cases reporting mainly benign clinical courses when treated early. Case presentation We present the case of a 67-year-old woman with progressive Zic4 antibody mediated PCD and rhombencephalitis. Immunomodulatory treatment, including intravenous methylprednisolone, plasmaphereses, and intravenous immunoglobulin (IVIG) was administered. Small cell lung cancer (SCLC) was detected, lobectomy performed and cyclophosphamide started. Despite this considerable therapeutic effort, rhombencephalitis led to defiant dysautonomia. Conclusion Paraneoplastic syndromes related to isolated Zic4 antibodies are rare and typically show a benign clinical course. Here, we present the first case of a rapidly progressive isolated Zic4 associated PCD and rhombencephalitis. Despite considerable therapeutic efforts, the patient passed away on autonomic dysfunction, highlighting the significance of Zic4 associated disease.

Extensive intrathoracic tumors are rarely diagnosed radiologically without pre-existing symptoms. If located in the posterior mediastinum, it is most probably a neurogenic tumor. Schwannoma is the most frequent neurogenic neoplasia in this location, and most schwannomas are benign. To specify the diagnosis, a thoracic computed tomography must be done; if the growth is close to the medullary canal, a magnetic resonance tomography of the spinal column is necessary to detect neuroforamen infiltration. Our surgical goal was complete excision of the tumor, although many authors favor a minimally invasive approach. In our patient we performed open, en bloc removal of the tumor; removal of parts of the intraforamen was also necessary, which necessitated revision of the affected neuroforamen. Histologically this was a very rare case of vagal schwannoma (which has an incidence of less than 6% of all neurogenic tumors). This patient has a very promising prognosis following complete tumor resection.

Background: Lung metastases can be removed by an Nd:YAG laser to save lung parenchyma. At these sites, a coagulated lung surface remains. Airtightness was investigated in relation to the depth of resection on an ex vivo porcine lung model. Methods: Freshly slaughtered porcine double lung preparations were connected to a ventilator via a tube. Non-anatomical laser resections were performed with an 800 μm laser fiber and the Nd:YAG laser LIMAX ® 120 (power: 40 and 60 watts). The following resection depths (each n=12) from the lung surface were examined: 0.5, 1, 1.5 and 2.0 cm. After resection the lungs were submerged under water and ventilated (frequency 10/min, P insp =25 mbar, PEEP =5 mbar). Airtightness of resection surfaces was determined by a leakage score, as well as the measurement of the leakage volume (in mL) per respiration (Group 1).Afterwards, the resection areas were coagulated for 5 seconds with a laser power of 60 watts at a distance of approximately 1 cm from the surface. This was followed by a re-evaluation for airtightness (Group 2).Finally, the resection surface was closed by a suture (PDS USP 4-0) and re-tested for airtightness (Group 3). The individual groups were compared for their significance (P<0.05) using a nonparametric test.Results: Up to a resection depth of 1.5 cm, the ventilated resection surfaces were completely airtight regardless of the laser power. From a depth of resection of 1.5 cm, a mean air volume loss of 28.9±5.3 mL/ respiratory cycle at 40 watts and of 26.4±5.8 mL at 60 watts was found. Additional surface coagulation did not significantly reduce the leakage rate. In contrast, suturing significantly reduced (P<0.0001) to 7.2±3.7 mL/ ventilation (40 watts) and 6.0±3.4 mL/ventilation. At a resection depth of 2 cm, the leakage volume was 42.9±3.3 mL/respiratory cycle (40 watt) and 46.3±6.4 mL/respiratory cycle (60 watt). Additional surface coagulation failed to significantly reduce leakage volume, but suture closure provided airtightness. Conclusions:In non-ventilated porcine lungs, Nd:YAG laser resection surfaces up to a resection depth of 1.5 cm are airtight after ventilation onset. From a depth of 1.5 cm, closure of resection surfaces by an additional suture is needed. Airtightness of resection surfaces was not increased by additional coagulation.