SummaryDonations after circulatory death (DCD) lung grafts are an alternative to extend the donor pool in lung transplantation. This study investigates the use of an in situ lung perfusion system (ISLP) in the donor to evaluate category I-II lungs. Pigs were sacrificed by ventricular fibrillation. All animals underwent 20 min of cardiopulmonary resuscitation and 5 min hands-off period after which heparin was administered. In group [WI-1], this was followed by 1 h of warm ischemia (WI) and 2 h of topical cooling (TC). In group [WI-2], 2 h of WI was followed by 1 h of TC. In group [WI-0], there was a minimal period of WI and no TC. In all three groups, the lungs were then evaluated during 60 min with ISLP. A high W/D weight ratio was correlated with a lower compliance, higher lactate production, and a higher PaCO 2 . ISLP is an effective way to assess the quality of lungs from category I-II DCD donors.

SummaryCurrent clinical lung preservation techniques have not eliminated ischaemiareperfusion (I/R) injury, despite many improvements. The optimal combination of flush and storage temperatures remain unclear in lung preservation. This is the first study to investigate a range of temperatures with 24-h inflated storage using consistent state-of-the-art preservation techniques. A rat lung transplant model was used to investigate the optimal combination of flush and storage temperatures. In six groups, rat lungs were flushed at 4°C, 10°C or room temperature (F 4 /F 10 /F Rt ) with Perfadex and stored inflated for 24 h in Perfadex on melting ice or at 10°C (S ice /S 10 ). Left donor lungs were transplanted for analysis. During 2-h reperfusion, the lung graft function was measured (blood gases, maximum ventilation pressure and static compliance) and lung graft injury was also assessed (W/ D ratio, total lung protein, Tryptase, Myeloperoxidase). Right donor lungs were assessed for W/D ratio only after flush and storage. For baseline measurements, left lungs without intervention were used. The combination of F Rt -S ice showed a significantly higher pO 2 , lower P max , low W/D ratios and total protein levels of left lungs after reperfusion when compared with F 4 -S ice and baseline. Storage at 10°C did not improve preservation. We conclude that F Rt -S ice creates the best lung graft preservation.

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CorrigendumIn [1], the reference list was ordered incorrectly. The below is the correct ordering of some of the references.