Currently, majority of the researchers concentrate on algal biomass production with autotrophic cultivation, however this cultivation strategy induces low biomass yield and it is troublesome to be utilized in large-scale algal biomass production. In contrary to this, heterotrophic algae can accumulate high level lipid production. Therefore, the present study was aimed to assess the effect of various carbon sources viz., glucose, sucrose, fructose, glycerol, sodium acetate and various nitrogen sources viz., NaNO3, urea, KNO3, NH4NO3, yeast extract, peptone, beef extract on lipid, biomass, total chlorophyll, protein and carbohydrate content in Scenedesmsus dimorphus. Among carbon sources, glucose showed maximum biomass yield (1.98±0.005gL-1) and highest lipid content (32.7±0.01%) followed by fructose, sucrose and glycerol. Similarly, total carbohydrates and protein content was also found to be maximum in glucose 0.275±0.002 mgmL-1 and 0.031±0.001 mgmL-1 respectively. While sodium nitrate supported maximum chlorophyll content (29.00±0.01 µgmL-1). Among various tested nitrogen sources, beef extract showed highest lipid production (30.28±0.05%), biomass yield (1.73±0.02 gL-1) in sodium nitrate and total carbohydrates (0.247±0.008) mgmL-1 in beef extract, followed by yeast extract and peptone. Highest chlorophyll content has been found in urea and maximum protein content in ammonium nitrate.
