This study investigated the dietary niche of the Barn Owl (Tyto alba) in an intensively farmed landscape, based on pellet samples from 12 nesting pairs containing 25 animal taxa and 1,994 prey items after the breeding season in 2016. Based on land use categories of the buffer area around each nest, three landscape types (agricultural, mosaic, urban) were considered, to analyse the diet composition and food-niche parameters. Niche breadth was calculated at the local and landscape level. Small mammals were the most frequent in the diet than other prey in each of the landscape types. The Common Vole (Microtus arvalis), considered to be an important agricultural pest was the most numerous prey in all landscape groups. The trophic niche of Barn Owl varied between 0.69 – 0.86 at the local level, and the overall value of niche breadth was significantly higher in the urban than in the other two landscape types. Our results showed that the increase of Common Vole frequency lead to a decrease in niche breadth; significantly negative relationship was detected between these parameters. Despite differences in niche breadth, similarly high niche overlaps were detected by the randomisation test in the three landscapes. Our results suggest that the diet composition of Barn Owls, mainly their food-niche pattern, reflected prey availability in the comparison of the studied landscapes, which pointed out that it is necessary to examine the dietary difference of Barn Owls at the finer scale of land use.

As an opportunistic predator, the Common Barn-owl (Tyto alba) proved to be an appropriate model organism to survey the composition of small mammal assemblages. This study analysed barn owls’ pellet samples from 14 localities containing 34 animal taxa and 4,088 prey items in two years (2015–2016). Two groups of samples (7–7 localities) were separated based on the dominance of semi-natural habitats and agricultural lands. Rarefaction analysis proved that the species richness and diversity of barn owls’ diet were significantly higher in semi-natural landscapes. The multiple regression analysis between PCA scores showed that in the agrarian landscape the abundance of generalist species was influenced by the proportion of forests, while the value of the trophic level index was determined by the size of arable fields. In the case of semi-natural landscapes, the abundance of the synantrop guild and generalist species, especially S. araneus and A. agrarius, was influenced by the proportion of urban areas, the number of habitats and the size of arable fields. The results of this study suggested that the small mammal consumption of the Common Barn-owl is significantly different in the two landscapes, which reflects the impact of habitat heterogeneity and agricultural activity on prey availability.