Medium amylose rice is a special type of rice that is highly demand by people in Asia and the world. Purpose of this study is to obtain individual F7 progeny (came from a cross between Sintanur x PTB33 and Pandanwangi x PTB33) with medium amylose content. Materials used were spectrophotometer with wave lenght 625 nm, meanwhile molecular markers applied were Wx. Results of this study showed that SP73-3-1-7 had usually most use the plant with medium amylose content (20-24%) based on molecular and phenotypic marker. The combination of both tests provides more accurate in selecting physicochemical trait in rice.