This study aims to delineate the tissue structure of male cat external reproductive organs using ultrasound with different types of consoles and transducers frequencies through indirect method. External organ of 5 tom weighing 2-3 kg was evaluated using ultrasound with stationary console (linear transducer, 10 MHz) and portable console (linear transducer, 6.5 MHz), transcutaneously. The results showed that both console and transducer could not visualize the structure of organs sized less than 10 mm through direct method. However, the indirect method using stationary console with a 10 MHz transducer enable to visualize the organ and depicts clearly the internal organ structure such as penis and its parts, scrotum and its constituent layers, caput and cauda of epididymis, and testicles. Furthermore, portable console with 6.5 MHz transducer was still able to provide an adequate image of those organs. In general, fibrous tissues such as tunica vaginalis, tunica Dartos, funiculusspermaticus, and urethra were visible in ultrasonogram as hyperechoic structures, while soft tissues such as the parts of testicles and penis were visible as hypoechoic structures. Tissue containing fluid such as urethral lumen was visible as anechoic structure. In conclusion, indirect ultrasound imaging method was sufficient to visualize the organs sized less than 10 mm using 3-15 MHz transducer in which was currently widely used in animal health care clinics.

Chemical castration is a method that can be applied easily without any surgical intervention in animals. This study utilized iron (III) chloride hexahydrate (FeCl 3 .6H 2 O) as a new material for chemical castration in mice. Twenty seven adult male mice were divided into five groups: FeCl 3 20% (n = 6), FeCl 3 10% (n = 6), FeCl 3 5.0% (n = 6), FeCl 3 2.5% (n = 6), and control NaCl 0.9% (n = 3). A 0.2 mL of NaCl 0.9% or FeCl 3 in various concentrations was injected intra-testicularly on each testis of the mice. Post-castration survival rate with LD 50 values was obtained at the concentrations between 2.5-5.0% of FeCl 3 groups, and 100% mice survived in the control group. The size of testis and concentration of spermatozoa decreased, in contrast with the increased concentration of FeCl 3 solution used seven days post-injection compared to the control group. ABSTRAKKebiri/kastrasi kimiawi secara injeksi intra-testis merupakan metode pengebiriam yang dapat dilakukan dengan mudah tanpa prosedur bedah pada hewan. Penelitian ini memanfaatkan larutan besi (ferri/III) klorida (FeCl 3 ) sebagai bahan baru untuk tindakan kebiri kimiawi pada mencit. Mencit jantan dewasa umur lima bulan sebanyak 27 ekor dibagi dalam lima kelompok yaitu FeCl 3 20% (n=6), FeCl 3 10% (n=6), FeCl 3 5,0% (n=6), FeCl 3 2,5% (n=6) dan kontrol NaCl 0,9% (n=3). Larutan FeCl 3 sebanyak 0,2 mL diinjeksikan secara intra-testikel pada setiap organ testis. Daya hidup pascakebiri injeksi nilai LD 50 diperoleh pada kelompok FeCl 3 konsentrasi di antara 2,5-5,0 % dan kelompok kontrol 100 % hidup. Organ testis dalam skrotum mengalami pengecilan ukuran dan konsentrasi spermatozoa mengalami penurunan seiring dengan peningkatan konsentrasi larutan FeCl 3 yang digunakan setelah tujuh hari pasca injeksi dibandingkan dengan kontrol.