Development of therapeutic agents against a model organism like P.aeruginosa, which forms biofilms, has been reviewed in the current study. A scientometric analysis has been used to predict the developments in Pseudomonas biofilm research, to configure potential antibiofilm agents (ABA) that can have prominent inhibition or dispersal effect and also overcome the problem of drug resistance. Diversity of ABA, antibiofilm strategy, prominent investigators and institutions in Pseudomonas biofilm research during the period 2009-2019 are the areas of focus, to understand the contributions in antibiofilm therapy. Scientometric study and the meta-analysis from 25,113 research publications, indicate nanoparticles as most promising control in biofilm based hazard. The results of investigation among majority of research groups reveal prominence of combinatorial strategy in targeting biofilms. A major shift from conventional antibiotics to nanoparticles as potential ABA is one of the significant findings of the study. Co-word analysis and Sankey plots were instrumental to identify the network of strategies focusing on biofilm inhibition. A Pearson correlation analysis for percentage of biofilm inhibition shows significant relevance with the nanoparticle type but not size. Moreover the impact of antibiofilm activity could be significantly correlated with source, nature and method of synthesis.

The Chromobacterium violaceum is a gram-negative facultative anaerobic bacterium that is known to cause human infections in lungs, liver, brain, spleen lymph nodes and urinary tract. It has Acyl Homoserine Lactone (AHL) regulated virulence features like violacein pigment production, swarming motility, biofilm formation and haemolysis. Bacterial pathogens form biofilms in natural as well as medical implants due to a complex signalling – “Quorum Sensing” (QS). QS builds an interaction among the cells, which increases the proliferation and mechanisms necessary for invasion into the host. Instead of using only bactericidal agents for infection control, suppression of QS by Quorum Quenching agents (QQ) can overcome limitations of currently used antimicrobial substances. In the present study biogenic silver nanoparticles (BSNPs) synthesized from selected five plant extracts were screened against Chromobacterium violaceum MCC 4212 for QQ potential. Biofilm inhibition of 91.8% and dispersal of 81.33% was found to be exhibited by BSNPsmade from extracts of Garcinia and Trachyspermum. Swarming nature was inhibited by 66% while there was complete inhibition of haemolysis by BSNPs. Therefore, the BSNPs synthesized were found potential to control the pathogenicity of C. violaceum 4212 as an antibiofilm agent.