The SELEX (Systematic Evolution of Ligands by EXponential enrichment) method has been used successfully since 1990, but work is still required to obtain highly specific aptamers. Here, we present a novel approach called ‘Competitive non-SELEX’ (and termed as ‘SELCOS’ (Systematic Evolution of Ligands by COmpetitive Selection)) for readily obtaining aptamers that can discriminate between highly similar targets. This approach is based on the theoretical background presented here, in which under the co-presence of two similar targets, a specific binding type can be enriched more than a nonspecifically binding one during repetitive steps of partitioning with no PCR amplification between them. This principle was experimentally confirmed by the selection experiment for influenza virus subtype-specific DNA aptamers. Namely, the selection products (pools of DNA aptamers) obtained by SELCOS were subjected to a DEPSOR-mode electrochemical sensor, enabling the method to select subtype-specific aptamer pools. From the clonal analysis of these pools, only a few rounds of in vitro selection were sufficient to achieve the surprisingly rapid enrichment of a small number of aptamers with high selectivity, which could be attributed to the SELCOS principle and the given selection pressure program. The subtype-specific aptamers obtained in this manner had a high affinity (e.g., K D = 82 pM for H1N1; 88 pM for H3N2) and negligible cross-reactivity. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal.

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Environmental pollution is becoming one of the major threats around the world because of the release of toxic and hazardous substances from food, pharmaceutical, and other industries as well. These wastes are mainly dumped indiscriminately which ultimately reached water bodies, thereby affecting marine ecosystem. Therefore, effective effluent treatment is an important step which can help in conserving our water resources. White rot fungus (WRF) have been shown to degrade and mineralize a wide variety of wastes because of their nonspecific extracellular lignin mineralizing enzymes (LMEs). These enzymes are used for the decolorization of synthetic dyes. They help in the degradation of pesticides, polycyclic aromatic hydrocarbons (PAHs), and pharmaceuticals wastes like- anti-inflammatory, lipid regulatory, antiepileptic drugs, endocrine disrupting chemicals, etc. They also help in degrading the food waste and convert them into useful products which can be used as food, feed, fodder; some of these wastes are lignocellulosic waste, viticulture waste, olive mill waste, molasses waste, etc.

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Laccases (E.C. 1.10.3.2 benzenediol: oxygen oxidoreductase) are an interesting group of N glycosylated multicopper blue oxidase enzymes and the widely studied enzyme having a broad range of substrate specificity of both phenolic and non-phenolic compounds. They are widely found in fungi, bacteria plant, insects, and in lichen. They catalyze the oxidation of various phenolic and non-phenolic compounds, with the concomitant reduction of molecular oxygen to water. They could increase productivity, efficiency, and quality of products without a costly investment. This chapter depicts the applications of laccase enzyme from white rot fungi, having various industrial (such as textile dye bleaching, paper and pulp bleaching, food includes the baking, it also utilized in fruit juice industry to improve the quality and stabilization of some perishable products having plant oils), pharmaceutical (as it has potential for the synthesis of several useful drugs such anticancerous, antioxidants, synthesis of hormone derivatives because of their high value of oxidation potential) significance.