United States Air Force (USAF) vision screening tests have remained largely unchanged since WWII and it is unclear whether current standards are applicable for users of new human-machine interfaces (e.g., stereoscopic remote vision system (RVS) in the refueling tanker). This study examined the relationships between several vision tests, including a set of automated vision tests (AVT) developed by the Operational Based Vision Assessment (OBVA)Laboratory, an electronic version of the standard Titmus stereo test, and the current Armed Forces Vision Tester (AFVT), and simulated air refueling task performance using a stereoscopic RVS. Additionally, the relationships between vision test scores and subjective measures of visual fatigue were analyzed. Results showed that AVT measures of disparity discrimination and horizontal fusion correlated with simulated air refueling performance. AVT measures of acuity, contrast sensitivity, disparity discrimination, and radial motion sensitivity were significantly associated with subjective measures of discomfort and visual fatigue. Notably, neither the electronic Titmus results, nor the AFVT measures were associated with either air refueling task performance or subjective measures of visual fatigue. Adjustments to the vision standards and test methods used for USAF aeromedical vision screening should therefore be considered.