The tetraploid germplasm of bahiagrass, Paspalum notatum Flüggé, is an unexploited source of variability that can be used for the genetic improvement of this species as forage or turf. The objectives of this research were to develop a segregating population by hybridizing induced sexual and apomictic clones and to characterize the resulting population for mode of reproduction, seed fertility, growth habit, seasonal growth pattern, and frost resistance. A population of 591 hybrids had a 4:1 ratio between sexual and apomictic (facultative + obligate apomictic) plants and an 8:1 ratio between others (facultative apomictic + sexual) and obligate apomictic plants. The general fertility of the progeny was higher than that of the induced sexual parents. Significant differences were observed for self‐ and cross‐fertility among apomictic progeny and for self‐fertility among sexual progeny. Most sexual progeny set the same amount of seed when self‐ or cross‐ pollinated, indicating an absence of self‐incompatibility. Marked variation was observed for growth habit, seasonal growth patterns, and frost resistance among the progeny. Heritability estimates obtained for these characteristics indicate that selection can be successfully used for the genetic improvement of this species.
Grasslands in warm‐climate regions are often based on grass monocultures, increasing their dependence on N fertilizers. Integrating perennial legumes into grass pastures is a logical option. The objective of this 2‐yr study was to assess seven rhizoma peanut (Arachis glabrata Benth) cultivars: Arbrook, Arblick, Ecoturf, Florigraze, Latitude 34, UF Peace, and UF Tito. Above‐ and belowground responses included biomass, in vitro organic matter disappearance (IVOMD), N concentration, N content, δ15N, proportion of N derived from atmosphere (%Ndfa), and biological N2 fixation (BNF). Arbrook was more productive than Florigraze in both years (P ≤ 0.05) but produced similar biomass to other varieties in 2014. In 2015, Arbrook also was more productive than Arblick and Latitude 34. Herbage N concentration ranged from 19.2 to 36.3 g kg−1. Arbrook tended to be less digestible than other rhizoma peanut cultivars. The BNF represented >80% of herbage N and averaged 200 kg N ha−1 yr−1, with values ranging from 123 to 280 kg N ha−1 yr−1. Root and rhizome biomass varied among cultivars, with Ecoturf (26.9 Mg organic matter [OM] ha−1) and Latitude 34 (27.8 Mg OM ha−1) presenting greater root and rhizome mass than Florigraze (10.5 Mg OM ha−1) but similar to other varieties. Roots and rhizomes represented a significant portion of the total biomass and N pool, and further studies are needed to assess turnover of these tissues as well as their N contribution in grazing systems using grass–rhizoma peanut mixtures.
Forage legumes increase nutritive value and provide N to grass‐based grazing systems. Few legumes have a long stand life in the southeastern US, but persistence is documented for rhizoma peanut (RP; Arachis glabrata Benth.). Several RP introductions have been released recently from the University of Florida, but their responses to grazing management have not been evaluated. The objective was to determine productivity, persistence, and nutritive value of three RP cultivars (‘Florigraze’, ‘UF Peace’, and ‘UF Tito’) and the germplasm Ecoturf grazed every 3 or 6 wk to remove 50 or 75% of pre‐grazing canopy height. Herbage accumulation (HA) was not different among RP entries and averaged 8790 and 6210 kg ha‐1 in Years 1 and 2, respectively. Greater HA occurred for the regrowth interval of 6 wk vs. 3 wk in the 50% removal treatment (8040 and 7010 kg ha‐1, respectively), and the response approached significance (P = 0.073) for the 75% treatment (7800 vs. 7140 kg ha‐1, respectively). Treatments had minimal effect on nutritive value, and all entries had crude protein (CP) ≥ 140 g kg‐1 and in vitro digestible organic matter (IVDOM) ≥ 660 g kg‐1. Grass encroachment was greater in Ecoturf and Florigraze when grazed every 3 wk (13 and 24%, respectively) than every 6 wk (7 and 15%, respectively), but regrowth interval did not affect grass percentage in Peace and Tito. New RP cultivars and germplasm had similar HA as Florigraze, but also greater percentage RP and lesser weed frequency than Florigraze, especially under frequent or close grazing.
Novel approaches are needed for overcoming barriers to successful association of herbaceous legumes with grasses in warm‐climate pastures and to identify low‐cost, long‐term solutions to the problem of N limitation in low‐input systems. The objective of this experiment was to evaluate defoliation management options during the year of establishment when rhizoma peanut (RP) (Arachis glabrata Benth.) was strip planted into existing bahiagrass (Paspalum notatum Flüggé). Treatments were four defoliation strategies: (i) Control (no defoliation of the planted RP strip and adjacent bahiagrass harvested for hay), (ii) Hay Production (RP strip and adjacent bahiagrass harvested for hay every 28 d), (iii) Simulated Continuous Stocking (pastures grazed weekly), and (iv) Rotational Stocking (pastures grazed every 28 d). Simulated Continuous and Rotational Stocking reduced RP canopy cover and frequency of occurrence. Greatest RP cover during the establishment year was achieved in August with 32 and 29% for the Control and Hay Production treatments compared to 5 and 4% for Simulated Continuous and Rotational Stocking, respectively. Spread of RP was least in Simulated Continuous Stocking. Light penetration to the level of RP in the canopy was not a primary driver of RP response because it was greatest for grazed plots where RP performed poorest. Results show that defoliation management during the establishment year is critical and if pastures are defoliated, hay production is the recommended option.
Moderate heat stress accompanied by short episodes of extreme heat during the post-anthesis stage is common in most US wheat growing areas and causes substantial yield losses. Sink strength (grain number) is a key yield limiting factor in modern wheat varieties. Increasing spike fertility (SF) and improving the partitioning of assimilates can optimize sink strength which is essential to improve wheat yield potential under a hot and humid environment. A genome-wide association study (GWAS) allows identification of novel quantitative trait loci (QTLs) associated with SF and other partitioning traits that can assist in marker assisted breeding. In this study, GWAS was performed on a soft wheat association mapping panel (SWAMP) comprised of 236 elite lines using 27,466 single nucleotide polymorphisms (SNPs). The panel was phenotyped in two heat stress locations over 3 years. GWAS identified 109 significant marker-trait associations (MTAs) (p ≤ 9.99 x 10−5) related to eight phenotypic traits including SF (a major component of grain number) and spike harvest index (SHI, a major component of grain weight). MTAs detected on chromosomes 1B, 3A, 3B, and 5A were associated with multiple traits and are potentially important targets for selection. More than half of the significant MTAs (60 out of 109) were found in genes encoding different types of proteins related to metabolism, disease, and abiotic stress including heat stress. These MTAs could be potential targets for further validation study and may be used in marker-assisted breeding for improving wheat grain yield under post-anthesis heat stress conditions. This is the first study to identify novel QTLs associated with SF and SHI which represent the major components of grain number and grain weight, respectively, in wheat.
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