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vone; 9.0 mg / kg, n ϭ 5) or saline (control group; n ϭ 5) at the same time as removing the clamps. Five hours after de-clamping, the muscles of lower extremity were harvested. Tissues were stained with hematoxylin & eosin (HE), in order to count the viable muscle cells. They were also stained with periodic acid-Schiff (PAS), in order to assess the glycogen storage in muscle cells.Results: The density of viable muscle cells in the group edaravone was significantly greater than that of control group (593Ϯ60 cells/mm2 vs. 258Ϯ31 cells/ mm2, P Ͻ .01). The mean percentage of PAS-positive area in the edaravone group was also significantly higher than that in control group (30.1 Ϯ 6.9 % vs. 7.3 Ϯ 2.1%, P Ͻ .001, Fig, original 200x).Conclusions: Our results suggest that edaravone injected at the start of reperfusion can also reduce muscle injury following leg ischemia in rats, storing a high level of glycogen in viable muscle cells. Therefore, edaravone might be useful in clinical settings following leg ischemia.