The goal of the presented study was to compare the proliferation of different U118 MG and U251 MG glioblastoma cell lines irradiated with proton beam or X-rays in dose range 0.5-10.0 Gy. Cytokinesis-block micronucleus (CBMN) assay was carried out to study changes in proliferation presented as nuclear division index (NDI). Preliminary results suggest that protons and X-rays influence GBM (glioblastoma multiforme) cellular proliferation differently. Therapeutically, a decrease in NDI values with the increase in both types of radiation dose was found only for U251 MG cell line, and thus can be classified as more radiosensitive than U118 MG cell line. Also for U251 MG GBM cell line, a therapeutic proton beam was more effective in inhibition of proliferation than X-rays. Genetic differences between GBMs are supposed to be involved in the increased radiosensitivity, which is planned to be studied further by gene expression analysis.

INTROduCTION Hereditary angioedema (HAE) is a rare autosomal dominant disease caused by genetic dysfunction of C1 inhibitor (C1-INH) due to mutations in the SERPING1 gene. The disorder is mediated mainly by bradykinin. The clinical course of the disease is varied and not related to genetic changes. OBjECTIvEs We aimed to evaluate redox homeostasis of peripheral blood mononuclear cells (PBMCs) in patients with HAE due to C1-INH deficiency (C1-INH-HAE) by measuring the levels of reactive oxygen species (ROS) of PBMCs as well as plasma advanced glycation end products (AGEs) and advanced oxidation protein products (AOPPs). We also aimed to assess the effect of bradykinin on ROS levels. PATIENTs ANd mEThOds We enrolled 30 adults with C1-INH-HAE and 15 healthy individuals. The levels of ROS were measured by flow cytometry, while the plasma levels of AGEs and AOPPs were determined spectrophotometrically by enzyme-linked immunosorbent assays. REsuLTs Basal and hydrogen peroxide (H 2 O 2)-induced ROS levels were higher in patients with HAE when compared with controls (P = 0.002 and P = 0.001, respectively), indicating abnormalities in redox homeostasis. Plasma AOPP and AGE levels were similar in both groups. Bradykinin reduced basal and H 2 O 2-induced ROS generation in PBMCs only in patients with HAE (P = 0.03). CONCLusIONs The higher basal and H 2 O 2-induced ROS levels in patients with C1-INH-HAE indicate redox imbalance. However, by reducing basal and H 2 O 2-induced ROS levels, bradykinin shows antioxidant action in this disorder.