Environmental omics and molecular-biological data have been proposed to yield improved quantitative predictions of biogeochemical processes. The abundances of functional genes and transcripts relate to the number of cells and activity of microorganisms. However, whether molecular-biological data can be quantitatively linked to reaction rates remains an open question. We present an enzyme-based denitrification model that simulates concentrations of transcription factors, functional-gene transcripts, enzymes, and solutes. We calibrated the model using experimental data from a well-controlled batch experiment with the denitrifier Paracoccous denitrificans. The model accurately predicts denitrification rates and measured transcript dynamics. The relationship between simulated transcript concentrations and reaction rates exhibits strong non-linearity and hysteresis related to the faster dynamics of gene transcription and substrate consumption, relative to enzyme production and decay. Hence, assuming a unique relationship between transcript-to-gene ratios and reaction rates, as frequently suggested, may be an erroneous simplification. Comparing model results of our enzyme-based model to those of a classical Monod-type model reveals that both formulations perform equally well with respect to nitrogen species, indicating only a low benefit of integrating molecular-biological data for estimating denitrification rates. Nonetheless, the enzyme-based model is a valuable tool to improve our mechanistic understanding of the relationship between biomolecular quantities and reaction rates. Furthermore, our results highlight that both enzyme kinetics (i.e., substrate limitation and inhibition) and gene expression or enzyme dynamics are important controls on denitrification rates.
Molecular-biological tools and so-called omics techniques, i.e., (meta)genomics, (meta)transcriptomics, (meta) proteomics analyses, have been used to characterize microbial reactions in various environments such as riparian
Molecular-biological tools and so-called omics techniques, i.e., (meta)genomics, (meta)transcriptomics, (meta) proteomics analyses, have been used to characterize microbial reactions in various environments such as riparian
The presence of anthropogenic organic micropollutants
in rivers
poses a long-term threat to surface water quality. To describe and
quantify the in-stream fate of single micropollutants, the advection–dispersion–reaction
(ADR) equation has been used previously. Understanding the dynamics
of the mixture effects and cytotoxicity that are cumulatively caused
by micropollutant mixtures along their flow path in rivers requires
a new concept. Thus, we extended the ADR equation from single micropollutants
to defined mixtures and then to the measured mixture effects of micropollutants
extracted from the same river water samples. Effects (single and mixture)
are expressed as effect units and toxic units, the inverse of effect
concentrations and inhibitory concentrations, respectively, quantified
using a panel of in vitro bioassays. We performed a Lagrangian sampling
campaign under unsteady flow, collecting river water that was impacted
by a wastewater treatment plant (WWTP) effluent. To reduce the computational
time, the solution of the ADR equation was expressed by a convolution-based
reactive transport approach, which was used to simulate the dynamics
of the effects. The dissipation dynamics of the individual micropollutants
were reproduced by the deterministic model following first-order kinetics.
The dynamics of experimental mixture effects without known compositions
were captured by the model ensemble obtained through Bayesian calibration.
The highly fluctuating WWTP effluent discharge dominated the temporal
patterns of the effect fluxes in the river. Minor inputs likely from
surface runoff and pesticide diffusion might contribute to the general
effect and cytotoxicity pattern but could not be confirmed by the
model-based analysis of the available effect and chemical data.
<p>Biomolecular quantities like gene, transcript or enzyme concentrations related to a specific reaction promise to provide information about the turnover of nutrients or contaminants in the environment. Particularly transcript-to-gene ratios have been suggested to provide a measure for reaction rates but a relationship with rates currently lacks validation.<br>We applied an enzyme-based reactive transport model for denitrification and aerobic respiration at the river-groundwater interface to simulate the temporal and spatial patterns of transcripts, enzymes and biomass under diurnal dissolved oxygen fluctuations.<br>Our analysis showed that transcript concentrations of denitrification genes exhibit considerable diurnal fluctuations, whereas enzyme concentrations and biomass are stable over time. The daily fluctuations in denitrification rates yielded a poor correlation between rates and transcript and enzyme concentrations. Daily averaged reaction rates, however, show a close-to-linear relationship with enzyme concentrations and mean transcript concentrations.<br>Our findings suggest that, under dynamic environmental conditions, single-event sampling may result in the misinterpretation of biomelucular quantities as these relate to reaction rates. A better representation of rates can be achieved via sampling that captures the temporal variability of a particular system.</p>
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