Identification of postoperative infections based on retrospective patient data is currently done using manual chart review. We used a validated, automated labelling method based on registrations and treatments to develop a high-quality prediction model (AUC 0.81) for postoperative infections.

Using only indirect, static markers of the dynamic cellular quality control system, autophagy, we have reported impaired autophagy in biopsied vascular endothelial cells (EC) of humans with aging that is related to endothelial dysfunction, as indicated by reduced endothelium‐dependent dilation (EDD), and oxidative stress. Here, we estimated dynamic autophagic flux in EC from 10 healthy adults aged 51‐73 by calculating the within‐subject difference in the expression of microtubule‐associated protein light chain 3 (LC3 ratio) between ECs incubated in the absence vs. presence of chloroquine (50 μM 1.5 hrs) to inhibit autophagy. EDD was assessed by: 1) brachial artery flow‐mediated dilation (FMD); and 2) forearm blood flow (FBF) to incremental brachial artery infusions of acetylcholine. Oxidative stress‐suppression of EDD was determined by ∆FBF to co‐infusion of vitamin C (vitC). The LC3 ratio was positively related to FMD (r=0.76, P<0.05) and FBF (r=0.56, P<0.05), but not endothelium‐independent dilation to NO donors (both P>0.05). The LC3 ratio also was inversely related to ∆FBF with vitC (r=‐0.57, P<0.05). These findings provide novel evidence that greater EC autophagic flux is associated with improved endothelial function linked to reduced oxidative stress in healthy middle‐aged/older adults. Grant Funding Source: NIH AG044031 TR001082 AG013038