a b s t r a c tThe description of the cell spatial pattern and characteristic distances is fundamental in a wide range of physio-pathological biological phenomena, from morphogenesis to cancer growth. Discrete particle models are widely used in this field, since they are focused on the cell-level of abstraction and are able to preserve the identity of single individuals reproducing their behavior. In particular, a fundamental role in determining the usefulness and the realism of a particle mathematical approach is played by the choice of the intercellular pairwise interaction kernel and by the estimate of its parameters. The aim of the paper is to demonstrate how the concept of H-stability, deriving from statistical mechanics, can have important implications in this respect. For any given interaction kernel, it in fact allows to a priori predict the regions of the free parameter space that result in stable configurations of the system characterized by a finite and strictly positive minimal interparticle distance, which is fundamental when dealing with biological phenomena. The proposed analytical arguments are indeed able to restrict the range of possible variations of selected model coefficients, whose exact estimate however requires further investigations (e.g., fitting with empirical data), as illustrated in this paper by series of representative simulations dealing with cell colony reorganization, sorting phenomena and zebrafish embryonic development.

In this article, we present a microscopic-discrete mathematical model describing crowd dynamics in no panic conditions. More specifically, pedestrians are set to move in order to reach a target destination and their movement is influenced by both behavioral strategies and physical forces. Behavioral strategies include individual desire to remain sufficiently far from structural elements (walls and obstacles) and from other walkers, while physical forces account for interpersonal collisions. The resulting pedestrian behavior emerges therefore from non-local, anisotropic and short/long-range interactions. Relevant improvements of our mathematical model with respect to similar microscopic-discrete approaches present in the literature are: (i) each pedestrian has his/her own dynamic gazing direction, which is regarded to as an independent degree of freedom and (ii) each walker is allowed to take dynamic strategic decisions according to his/her environmental awareness, which increases due to new information acquired on the surrounding space through their visual region. The resulting mathematical modeling environment is then applied to specific scenarios that, although simplified, resemble real-word situations. In particular, we focus on pedestrian flow in twodimensional buildings with several structural elements (i.e., corridors, divisors and columns, and exit doors). The noticeable heterogeneity of possible applications demonstrates the potential of our mathematical model in addressing different engineering problems, allowing for optimization issues as well.Communicated by Eduardo Souza de Cursi.

This paper deals with the derivation of a collective model of cell populations out of an individual-based description of the underlying physical particle system. By looking at the spatial distribution of cells in terms of time-evolving measures, rather than at individual cell paths, we obtain an ensemble representation stemming from the phenomenological behavior of the single component cells. In particular, as a key advantage of our approach, the scale of representation of the system, i.e., microscopic/discrete vs. macroscopic/continuous, can be chosen a posteriori according only to the spatial structure given to the aforesaid measures. The paper focuses in particular on the use of different scales based on the specific functions performed by cells. A two-population hybrid system is considered, where cells with a specialized/differentiated phenotype are treated as a discrete population of point masses while unspecialized/undifferentiated cell aggregates are represented by a continuous approximation. Numerical simulations and analytical investigations emphasize the role of some biologically relevant parameters in determining the specific evolution of such a hybrid cell system.

Biological systems are typically formed by different cell phenotypes, characterized by specific biophysical properties and behaviors. Moreover, cells are able to undergo differentiation or phenotypic transitions upon internal or external stimuli. In order to take these phenomena into account, we here propose a modelling framework in which cells can be described either as pointwise/concentrated particles or as distributed masses, according to their biological determinants. A set of suitable rules then defines a coherent procedure to switch between the two mathematical representations. The theoretical environment describing cell transition is then enriched by including cell migratory dynamics and duplication/apoptotic processes, as well as the kinetics of selected diffusing chemicals influencing the system evolution. Finally, biologically relevant numerical realizations are presented: in particular, they deal with the growth of a tumor spheroid and with the initial differentiation stages of the formation of the zebrafish posterior lateral line. Both phenomena mainly rely on cell phenotypic transition and differentiated behaviour, thereby constituting biological systems particularly suitable to assess the advantages of the proposed model.

The swarming of a bee colony is guided by a small group of scout individuals, which are informed of the target destination (the new nest). However, little is known on the underlying mechanisms, i.e. on how the information is passed within the population. In this respect, we here present a discrete mathematical model to investigate these aspects. In particular, each bee, represented by a material point, is assigned its status within the colony and set to move according to individual strategies and social interactions. More specifically, we propose alternative assumptions on the flight synchronization mechanism of uninformed individuals and on the characteristic dynamics of the scout insects. Numerical realizations then point out the combinations of behavioural hypotheses resulting in collective productive movement. An analysis of the role of the scout bee percentage and of the phenomenology of the swarm in domains with structural elements is finally performed.