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This study looked at the feasibility of targeting the zona pellucida for a contraceptive vaccine as a possible alternative method of control for overabundant macropods. Tammar wallabies, as a model for other macropods, were immunized with porcine zonae pellucidae (PZP) and were found to achieve significant concentrations of antibody to PZP in sera and reproductive tract fluids. Wallabies immunized with PZP exhibited lower ovarian weight with reduced numbers of antral follicles when compared with control animals. Wallabies were placed in a natural mating trial followed by an artificial insemination trial. None of the PZP-immunized wallabies produced offspring in the natural mating trial compared with 67% of control animals. To further assess fertility, a sub-sample of the wallabies were superovulated and artificially inseminated. This resulted in all control wallabies producing fertilized ova and all PZP-immunized wallabies failing to ovulate. These results suggest that immunocontraception based on targeting antigens of the zona pellucida may be an effective strategy for fertility reduction in macropods.

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In the present study, male and female tammar wallabies were immunised with whole tammar wallaby sperm in adjuvant. An assay for sperm antibodies using a live sperm ELISA has been developed to detect sperm surface antigens and used to validate an assay using a 3-[(3-cholamidopropyl) dimethylammonio]-1 propanesulfonate (CHAPS) membrane extract of whole tammar wallaby sperm. The tests were used to monitor the immune response to whole sperm in both male and female tammar wallabies. Antisera with a limited array of specificities were generated, with those locating to the midpiece region of the sperm appearing the most likely candidates for targets for fertility perturbation based on immunofluorescence of fixed and non-fixed sperm. These systemically generated antibodies were demonstrated to have access to both the female and male tammar reproductive tracts and were found on ejaculated sperm and antibodies from female sera and follicular fluid-labelled fresh ejaculated sperm from non-immunised males. Preliminary sequencing of these proteins has identified some possibilities for further investigation.