-Introduction. Beginning in 1994, vanilla vines grown under shade houses in Reunion Island have developed disorders mainly due to the cymbidium mosaic potexvirus (CymV) and potyviruses. To reduce damage and avoid viral epidemics, we considered developing a vanilla nursery to supply farmers with virus-free plant material and to provide prophylactic advice. Accordingly, we determined the relationship between symptoms on vanilla leaves and viral diagnosis. We also determined the temporal and spatial distribution patterns of viral diseases. Materials and methods. Symptom assessment and serological tests were carried out on vanilla leaves (Vanilla fragrans) at various times. The relationship between symptoms and viral infection data was analyzed with Statistica software. Spatial distribution patterns of infected plants were generated with two-dimensional distance class analysis (2DClass). Results and discussion. Results showed that symptoms do not correlate well with serological diagnosis. Plants in all shade houses in the survey were infected mainly by CymV which spreads in the row according to cultural practices. Potyviruses could be transmitted both by insect vectors and mechanically, but for the latter case less efficiently than CymV. Conclusion.Results from this study can be used to develop control measures, including the production of healthy planting materials to manage plant disease epidemics. Further studies will involve the characterization of vanilla potyviruses so that reliable serological techniques and better molecular tools can be developed. Reunion / Vanilla fragrans / disease surveys / protected cultivation / potexviruses / potyviruses / symptoms / disease transmission / epidemiologyDissémination sous ombrière du cymbidium mosaic potexvirus et de potyvirus affectant les lianes de vanilliers cultivés à l'île de la Réunion.Résumé -Introduction. Depuis 1994, le dépérissement du vanillier, cultivé en système intensif sous ombrière à l'île de la Réunion, est principalement dû au « cymbidium mosaic virus » (CymV) et aux potyvirus. Pour limiter les dégâts et mieux contrôler l'épidémie virale, nous avons étudié la relation entre la présence de symptômes et le diagnostic viral ainsi que la dynamique temporelle et spatiale des virus sous ombrière. Ces études ont été utilisées pour apporter de judicieux conseils prophylactiques et mettre en place une pépinière de boutures de vanillier afin de fournir aux planteurs du matériel végétal indemne de virus. Matériel et méthodes. L'observation des symptômes et le diagnostic sérologique ont été réalisés à partir de feuilles de vanille (Vanilla fragrans) à l'occasion de différentes prospections sous ombrières. La relation entre l'expression des symptômes et les données d'infection virale a été analysée avec le logiciel Statistica. La distribution spatiale des lianes infectées a été étudiée par la méthode d'analyse des classes de distance en deux dimensions (2Dclass). Résultats et discussion. Les résultats montrent que l'expression des symptômes n'est pas systém...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.