Three bis(dithiocarbamate) derivatives of glycerol were obtained in 30−95% yields by reaction of a dithiocarbamic acid salt with 1,3-dichloro-1,3-dideoxyglycerol. The in vitro antifungal activity of the new compounds was evaluated against Alternaria brassicae, Pseudocercosporella herpotrichoides, Septoria nodorum, and Phytophtora cinnamomi. Some of the compounds displayed greater activity than commercial fungicides. Bis(N,N-diethyldithiocarbamoyl)-1,3-dideoxyglycerol (4) was found to maintain its pesticidal activity up to 24 days in open field tests against A. brassicae. Keywords: Fungicides; bis(1,3-dithiocarbamoyl)-1,3-dideoxyglycerol; in vitro antifungal activity; in vivo antifungal activity; open field test