BACKGROUND All parts of skeletal system are held together and moved by skeletal muscles, which provide for locomotion for the individual to survive. Four pairs of muscles make a group of muscles of mastication: temporalis, masseter, medial and lateral pterygoid. Masseter is a rectangular muscle originating from the zygomatic arch extending downwards to lateral aspect of lower border of ramus. We wanted to evaluate the activity of masseter and temporalis muscle using electromyogram in subjects with balanced and non-balanced occlusion complete dentures. METHODS 38 participants were selected and grouped into two as balanced and non-balanced occlusal denture subjects. Steps of fabricating a complete denture was followed along by addition of tracing in cases of balanced occlusion group. This was followed by recording the electromyographic readings at 1st appointment followed by an interval of 1 week and 3 weeks. These observations were then evaluated and compared for masseter and temporalis between the study groups. RESULTS The results show that there was an improvement in the muscle activity of temporalis and masseter when balanced group was observed following a period of 48 hours. However, the masseter showed noticeable improvement in non-balanced group at the end of 3 months when compared to balanced group. CONCLUSIONS This study was conducted under the hypothesis that there will be no change in the EMG activity of masseter and temporalis muscles in patients wearing complete dentures with and without balanced occlusion. Although, it was not statistically significant, the EMG recordings of masseter and temporalis, amongst balanced and non-balanced group, had a notable variance in the recordings to support that balanced occlusal scheme does not have a complete influence over all masticatory muscles.