Work stress on nurses is one of the problems in human resource management in hospitals. With the increasing work demands, the more likely nurses are to experience work stress, especially during the Covid-19 pandemic. This study aims to determine the relationship between workload and working hours with the work stress of nurses during the Covid-19 pandemic in the inpatient room of the Kendari City Hospital. This study method is an observational quantitative with a cross-sectional study design and using the Chi-square test. The population is 122 nurses and a total samples of 55 people that is obtained by using proportional random sampling technique for sampling. The instrument used is a questionnaire. The results showed that there was a relationship between workload and work stress with a p-value of 0.026 and there was a relationship between working hours and work stress with a p value of 0.035. The conclusion of this study was that the work stress of nurses in the inpatient room at the of the General Hospital Kendari City was influenced by workload and working hours.