Peptides are the building blocks which are widely used owing to their biology as well as their chemistry. They provide a vast platform in the area of medicine. Self-assembling peptides are peptide biomaterials which are pacing in the field of diagnosis, therapeutics, tissue regeneration and vaccine. Self-assembling peptides provide an excellent alternative to the conventional methods for the drug delivery and the treatment. In this article, we discuss about the various medical applications of self-assembling peptide as they have excellent biocompatibility and resemblance with the proteins in the biological system. These are constructed and modified using various amino acid sequences depending upon the type of the application for which it is being used