Abstract. Segmentation is a best method to divide the required region from the medical images. This research is based on segmentation of medical images (MRI, CT scans) based on the previous method known as pre-operative and post-recurrence tumor registration (PORTR) and proposed method biography based neural clustering (BBNC) with genetic processing for tumor segmentation. By using the new technique the extracted part can be view in 3D model and also can get the actual segmented tumor region. This new method will be helpful for diagnostics to find the tumor area as well as pixel difference in segmented part to define the tumor area accurately. While in the previous approach all the parameters have been used likewise, in which the registration method is used to transform the different sets of data into one coordinate system for segmentation of medical images. Registration basically is used to improve the signals to reduce the noise from the images. These techniques are better to find the tumor area from the MRI and CT scans, but after comparing them better results have been obtained in proposed technique. The proposed technique (BBNC) reduces the extracted region again into required and actual region of tumor with accuracy of area, time and pixel difference.