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Honey adulteration is a common practice that affects food quality and sale prices, and certifying the origin of the honey using non-destructive methods is critical. Guindo Santo and Quillay are fundamental for the honey production of Biobío and the Ñuble region in Chile. Furthermore, Guindo Santo only exists in this area of the world. Therefore, certifying honey of this species is crucial for beekeeper communities—mostly natives—to give them advantages and competitiveness in the global market. To solve this necessity, we present a system for detecting adulterated endemic honey that combines different artificial intelligence networks with a confocal optical microscope and a tunable optical filter for hyperspectral data acquisition. Honey samples artificially adulterated with syrups at concentrations undetectable to the naked eye were used for validating different artificial intelligence models. Comparing Linear discriminant analysis (LDA), Support vector machine (SVM), and Neural Network (NN), we reach the best average accuracy value with SVM of 93% for all classes in both kinds of honey. We hope these results will be the starting point of a method for honey certification in Chile in an automated way and with high precision.

We propose a 3D full-field focusing method for microscopic mid-wave infrared (MWIR) imagery. The method is based on the experimental estimation of a confined volumetric vision microscope point spread function. The technique employs our well-known constant-range-based nonuniformity correction algorithm as a preprocessing step and then an iteration in the z -axis Fourier-based deconvolution. The technique’s ability to compensate for localized blur is demonstrated using two different real MWIR microscopic video sequences, captured from two microscopic living organisms using a Janos-Sofradir MWIR microscopy setup. The performance of the proposed algorithm is assessed on real and simulated noisy infrared data by computing the root-mean-square error and the roughness Laplacian pattern indexes, which are specifically developed for the present work.

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A reference-free image index to jointly assess infrared-imaging fixed-pattern-noise and blur artifacts is proposed in this work. The proposed index is based on tuned-spatial-domain filtering, which works by combining two Laplace operators to simultaneously quantify the global infrared-imaging fixedpattern-noise and the global or local blur artifacts. The index effectiveness is demonstrated by two task-based image-quality assessments to determine the focused and fixed-pattern-noise free images from sequences captured with both a mid-wave-infrared microscope system and a long-wave-infrared plenoptic system. The index quantitative limits are shown on numerical computations over synthetic corrupted images as well as real black-body radiator calibrated infrared images with representative simulated fixed-pattern noise, from six well known infrared focal plane arrays transducer technologies, along with artificial blur added using real infrared imaging system point spread functions.