The removal of hydrocarbon compounds from the environment has always been a difficult undertaking for people all over the world. As a result, remedial efforts are required to safeguard the environment as well as to restore agriculture. This study looked at how poultry manure (dung) could help in the bioremediation of Veritas University's intentionally crude oil polluted soil. The physical properties of the soil and total petroleum hydrocarbon content of the crude oil polluted soil were determined, followed by the isolation and identification of microorganisms present in the soil and poultry manure before and after pollution with crude oil. These analyses were done according to standard operating procedures. Within a six-month timeframe, the natural attenuation and poultry manure amendment option for remediating the crude oil-polluted soil were monitored and the residual hydrocarbon content of the polluted soil after remediation evaluated. There was an overall decrease in pH level during the experimental units containing 4 g amendment, 2 g amendment and the untreated polluted soil in the order 8.4 to 7.1, 8.4 to 7.2 and 8.1 to 6.7, respectively. Temperature was within 27oC and 31oC, the treated soil that contained 4 g dung had an initial moisture content of 58%, and 35% after remediation. The hydrocarbon utilizing bacteria isolated were Pseudomonas species, Staphylococcus species, and Bacillus species, while the hydrocarbon utilizing fungi isolated were Aspergillus niger, Fusarium solani and Candida albicans. There was more significant microbial increase in the sample containing 4 g poultry manure treatment than 2 g poultry manure treatment and natural attenuation. Total hydrocarbon quantity significantly decreased after six months with the complete removal of C4, C7, C19 and C37 from the treated sample containing 4 g poultry manure. This indicated that the negative effects of crude oil on the environment can be mitigated by adding poultry manure.

Mishandling of petroleum products has been a significant source of environmental pollution and health hazards. Many microorganisms have the ability to utilize hydrocarbons as the sole source of carbon and these microorganisms are widely distributed in nature. Five (5) sites in Keffi, Nigeria were sampled to isolate hydrocarbon utilizing bacteria and fungi. The hydrocarbon products utilized were petrol, gear oil and engine oil. The hydrocarbon utilization was determined using the spectrophotometric method. A total of two (2) bacteria and two (2) fungi species were identified as the highest utilizers of the hydrocarbon products. The hydrocarbon utilization rate at the best temperature (37OC), pH (7) and time (28 days) was assessed. The utilization (mg/ml) of Pseudomonas aeruginosa for petrol ranges from 1.97±0.05, 1.31±0.034 for gear oil and 1.52±0.035 for engine oil. Alcaligenes faecalis utilization ranges from 2.2±0.022 for petrol, 1.57±0.031, for gear oil and 1.86± 0.034 for engine oil. Trichoderma harzianum ranges from 1.98 ±0.012 for petrol, 1.23±0.003 for gear oil and 1.73±0.008 for engine oil. Purpureocillium lilacinum ranges from 1.98±0.03 for petrol, 0.92±0.006 for gear oil and 1.39±0.035 for engine oil. The effect of microbial consortium on hydrocarbon utilization (mg/ml) for Pseudomonas aeruginosa and Alcaligenes faecalis ranges from 1.83 ±0.035 for petrol, 1.33 ± 0.023 for gear oil and 1.46 ± 0.015 for engine oil. Trichoderma harzianum and Purpureocillium lilacinum range from 1.88 ± 0.041 for petrol, 1.45 ± 0.026 for gear oil and 1.63 ± 0.011 for engine oil. While a microbial consortium of Pseudomonas aeruginosa, Alcaligenes faecalis, Trichoderma harzianum and Purpureocillium lilacinum ranges from 2.09 ± 0.002 for Petrol, 1.85 ± 0.031 for gear oil and 1.97 ± 0.034 for engine oil. P.aeruginosa, A. faecalis, T. harzianum and P. lilacinum constitute an effective microbial consortium for the bioremediation of hydrocarbon polluted soil.