Venoms can deleteriously affect any physiological system reachable by the bloodstream, including directly interfering with the coagulation cascade. Such coagulopathic toxins may be anticoagulants or procoagulants. Snake venoms are unique in their use of procoagulant toxins for predatory purposes. The boomslang (Dispholidus typus) and the twig snakes (Thelotornis species) are iconic African snakes belonging to the family Colubridae. Both species produce strikingly similar lethal procoagulant pathologies. Despite these similarities, antivenom is only produced for treating bites by D. typus, and the mechanisms of action of both venoms have been understudied. In this study, we investigated the venom of D. typus and T. mossambicanus utilising a range of proteomic and bioactivity approaches, including determining the procoagulant properties of both venoms in relation to the human coagulation pathways. In doing so, we developed a novel procoagulant assay, utilising a Stago STA-R Max analyser, to accurately detect real time clotting in plasma at varying concentrations of venom. This approach was used to assess the clotting capabilities of the two venoms both with and without calcium and phospholipid co-factors. We found that T. mossambicanus produced a significantly stronger coagulation response compared to D. typus. Functional enzyme assays showed that T. mossambicanus also exhibited a higher metalloprotease and phospholipase activity but had a much lower serine protease activity relative to D. typus venom. The neutralising capability of the available boomslang antivenom was also investigated on both species, with it being 11.3 times more effective upon D. typus venom than T. mossambicanus. In addition to being a faster clotting venom, T. mossambicanus was revealed to be a much more complex venom composition than D. typus. This is consistent with patterns seen for other snakes with venom complexity linked to dietary complexity. Consistent with the external morphological differences in head shape between the two species, CT and MRI analyses revealed significant internal structural differences in skull architecture and venom gland anatomy. This study increases our understanding of not only the biodiscovery potential of these medically important species but also increases our knowledge of the pathological relationship between venom and the human coagulation cascade.

The collection and dissemination of vertebrate ichnological data is struggling to keep up with techniques that are becoming commonplace in the wider palaeontological field. A standard protocol is required to ensure that data is recorded, presented and archived in a manner that will be useful both to contemporary researchers, and to future generations. Primarily, our aim is to make the 3D capture of ichnological data standard practice, and to provide guidance on how such 3D data can be communicated effectively (both via the literature and other means) and archived openly and in perpetuity. We recommend capture of 3D data, and the presentation of said data in the form of photographs, false‐colour images, and interpretive drawings. Raw data (3D models of traces) should always be provided in a form usable by other researchers (i.e. in an open format). If adopted by the field as a whole, the result will be a more robust and uniform literature, supplemented by unparalleled availability of datasets for future workers.

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Extensive and well-preserved tracksites in the coastally exposed Lower Cretaceous (Valanginian-Barremian) Broome Sandstone of the Dampier Peninsula provide almost the entire fossil record of dinosaurs from the western half of the Australian continent. Tracks near the town of Broome were described in the late 1960s as Megalosauropus broomensis and attributed to a medium-sized theropod trackmaker. Brief reports in the early 1990s suggested the occurrence of at least another nine types of tracks, referable to theropod, sauropod, ornithopod, and thyreophoran trackmakers, at scattered tracksites spread over more than 80 km of coastline north of Broome, potentially representing one of the world's most diverse dinosaurian ichnofaunas. More recently, it has been proposed that this number could be as high as 16 and that the sites are spread over more than 200 km. However, the only substantial research that has been published on these more recent discoveries is a preliminary study of the sauropod tracks and an account of the ways in which the heavy passage of sauropod trackmakers may have shaped the Dampier Peninsula's Early Cretaceous landscape. With the other types of dinosaurian tracks in the Broome Sandstone remaining undescribed, and the full extent and nature of the Dampier Peninsula's dinosaurian tracksites yet to be adequately addressed, the overall scientific significance of the ichnofauna has remained enigmatic.At the request of the area's Goolarabooloo Traditional Custodians, 400C hours of ichnological survey work was undertaken from 2011 to 2016 on the 25 km stretch of coastline in the Yanijarri-Lurujarri section of the Dampier Peninsula, inclusive of the coastline at Walmadany (James Price Point). Forty-eight discrete dinosaurian tracksites were identified in this area, and thousands of tracks were examined and measured in situ and using three-dimensional photogrammetry. Tracksites were concentrated in three main areas along the coast: Yanijarri in the north, Walmadany in the middle, and Kardilakan-Jajal Buru in the south. Lithofacies analysis revealed 16 repeated facies types that occurred in three distinctive lithofacies associations, indicative of an environmental transgression between the distal fluvial to deltaic portions of a large braid plain, with migrating sand bodies and periodic sheet floods. The main dinosaurian track-bearing horizons seem to have been generated between periodic sheet floods that blanketed the preexisting sand bodies within the braid plain portion of a tidally influenced delta, with much of the original, gently undulating topography now preserved over large expanses of the present day intertidal reef system. Of the tracks examined, 150 could be identified and are assignable to a least eleven and possibly as many as 21 different track types: five different types of theropod tracks, at least six types of sauropod tracks, four types of ornithopod tracks, and six types of thyreophoran tracks. Eleven of these track types can formally be assigned or compared to existing or new ichnotaxa,...